| 产品编号 | bs-8555R |
| 英文名称 | Desmuslin Rabbit pAb |
| 中文名称 | 中间丝蛋白β-synemin抗体 |
| 别 名 | Desmuslin; DMN; SYN; Synemin alpha; Synemin beta; Synemin, intermediate filament protein; SYNM; KIAA0353; synemin isoform A; SYNEM_HUMAN. |
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Specific References (4) | bs-8555R has been referenced in 4 publications.
[IF=15.887] Weihl, Conrad C.. et al. Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure. ACTA NEUROPATHOL. 2022 Oct;:1-17 WB ; Mouse.
[IF=6.39] Bengoechea, Rocio, et al. "Myofibrillar disruption and RNA binding protein aggregation in a mouse model of limb girdle muscular dystrophy 1D." Human Molecular Genetics (2015): ddv363. WB ; Mouse.
[IF=6.183] Jipeng Jiang. et al. 3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine. Biomater Sci-Uk. 2020 Nov;8(22):6362-6374 IF ; Dog.
[IF=4.902] Bengoechea et al. Myofibrillar disruption and RNA-binding protein aggregation in a mouse model of limb-girdle muscular dystrophy 1D. (2015) Hum.Mol.Genet. 24:6588-602 WB ; Mouse.
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| 研究领域 | 细胞生物 信号转导 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 172 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Desmuslin: 501-650/1565 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is an intermediate filament (IF) family member. IF proteins are cytoskeletal proteins that confer resistance to mechanical stress and are encoded by a dispersed multigene family. This protein has been found to form a linkage between desmin, which is a subunit of the IF network, and the extracellular matrix, and provides an important structural support in muscle. Two alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Function: Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. Subunit: Interacts with GFAP and VIM (By similarity). Isoform 1 interacts with TLN1 and VCL. Isoform 2 interacts with DES and DTNA. Isoform 1 and isoform 2 interact with DMD and UTRN. Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Tissue Specificity: Isoform 2 is strongly detected in adult heart, fetal skeletal muscles and fetal heart. Isoform 1 is weakly detected in fetal heart and also in fetal skeletal muscle. Isoform 1 and isoform 2 are detected in adult bladder (at protein level). The mRNA is predominantly expressed in heart and muscle with some expression in brain which may be due to tissue-specific isoforms. Similarity: Belongs to the intermediate filament family. SWISS: O15061 Gene ID: 23336 Database links: Entrez Gene: 23336 Human Entrez Gene: 233335 Mouse Omim: 606087 Human SwissProt: O15061 Human SwissProt: Q70IV5 Mouse Unigene: 207106 Human Unigene: 60526 Mouse Unigene: 46362 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Desmuslin) Polyclonal Antibody, Unconjugated (bs-8555R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
