| 产品编号 | bs-8689R |
| 英文名称 | MEPE Rabbit pAb |
| 中文名称 | 细胞外基质磷酸化抗体 |
| 别 名 | Matrix extracellular phosphoglycoprotein; MEPE; MEPE_HUMAN; OF45; Osteoblast/osteocyte factor 45. |
 |
Specific References (1) | bs-8689R has been referenced in 1 publications.
[IF=3.423] Baris Ozgur Donmez. et al. Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes. MOL MED REP. 2022 Dec;26(6):1-11 WB ; Mouse.
|
| 研究领域 | 免疫学 发育生物学 信号转导 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MEPE: 201-300/525 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MEPE is a 525 amino acid extracellular matrix protein. Expressed in osteocytes and brain, MEPE is a regulator of bone metabolism that is thought to mediate mineralization and demineralization within the osteocyte microenvironment. MEPE contains an RGD cell-attachment motif and shares molecular similarities with several dentin-bone extracellular matrix RGD-containing phosphoglycoproteins, including OPN (osteopontin) and DSP (dentin sialophosphoprotein). Via its ability to control bone mineralization, MEPE is associated with various developmental events such as skeletogenesis, bone regeneration and odontogenesis. MEPE is secreted in hypophosphatemic osteomalacia tumors, suggesting a possible role in the pathophysiology of bone-related cancers. Function: Seems to play a role in mineralization. Subcellular Location: Secreted Tissue Specificity: Expressed by osteoblasts. Secreted from oncogenic hypophosphataemic tumors. Post-translational modifications: Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. SWISS: Q9NQ76 Gene ID: 56955 Database links: Entrez Gene: 56955 Human Entrez Gene: 94111 Mouse Omim: 605912 Human SwissProt: Q9NQ76 Human Unigene: 676357 Human Unigene: 196672 Mouse Unigene: 160717 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Spleen tissue lysates
Lane 4: Mouse Cerebellum tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human MCF-7 cell lysates
Primary: Anti-MEPE (bs-8689R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 47,62 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
