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Rabbit Anti-MRF/C11orf9  antibody (bs-11191R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-11191R
英文名称 MRF/C11orf9
中文名称 髓鞘基因调控因子抗体
别    名 C11orf9; Chromosome 11 open reading frame 9; Gm1804; gm98; KIAA0954; MGC10781; MRF; MRF_HUMAN; Myelin gene regulatory factor; Ndt80.  
Specific References  (1)     |     bs-11191R has been referenced in 1 publications.
[IF=3.47] Xiaowei Yu. et al. Nanophthalmos-Associated MYRF Gene Mutation Causes Ciliary Zonule Defects in Mice. Invest Ophth Vis Sci. 2021 Mar;62(3):1-1  WB,IHC ;  Mouse.  
研究领域 细胞生物  免疫学  神经生物学  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 124kDa
细胞定位 细胞核 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MRF: 401-500/1151 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 C11orf9 (chromosome 11 open reading frame 9), also known as MRF (myelin gene regulatory factor), Ndt80, KIAA0954 or MGC10781, is a 1,151 amino acid single-pass membrane protein that localizes to nucleus. A member of the MRF family, C11orf9 is expressed in the ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. C11orf9 is weakly expressed in cerebellum and retina. C11orf9 contains one NDT80 DNA-binding domain and acts as a transcription factor that is required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. C11orf9 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11, which consists of approximately 135 million base pairs and 1,400 genes. Chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome.

Function:
Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.

Subunit:
Belongs to the MRF family. Contains 1 NDT80 DNA-binding domain.

Subcellular Location:
Membrane. Nucleus.

Tissue Specificity:
Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.

Similarity:
Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.

SWISS:
Q9Y2G1

Gene ID:
745

Database links:

Entrez Gene: 225908 Mouse

SwissProt: Q3UR85 Mouse

Unigene: 329716 Mouse



产品图片
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-MRF/C11orf9 (bs-11191R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U2OS cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(bs-11191R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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