| 产品编号 | bs-11212R |
| 英文名称 | KLC1 Rabbit pAb |
| 中文名称 | 驱动蛋白2抗体 |
| 别 名 | Kinesin 2 60/70kDa; Kinesin light chain 1; Kinesin2; Kinesin-2; KLC 1; KLC; KLC1; KLC1_HUMAN; KNS 2; KNS 2A; KNS2; KNS2A; Medulloblastoma antigen MU MB 2.50; MGC15245. |
| 研究领域 | 神经生物学 信号转导 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 65 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLC1: 1-100/573 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The kinesin family of motor proteins comprise at least two forms of conventional kinesin (kinesin-I). They are encoded by different genes and designated ubiquitous kinesin, which is expressed in all cells and tissues, and neuronal kinesin, which is expressed exclusively in neuronal cells. Conventional kinesin is a heterotetramer of two kinesin heavy chain subunits and two kinesin light chain subunits. While the kinesin heavy chain contains motor activity, evidence suggests that the kinesin light chain is involved in either modulation of kinesin heavy chain activity or in cargo binding. The motor protein kinesin is a heterotetramer composed of two heavy chains and two light chains. Kinesin motor activity is dependent on the presence of ATP and microtubules. Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. Subunit: Belongs to the kinesin light chain family. Contains 6 TPR repeats. Subcellular Location: Cytoplasm; cytoskeleton. Tissue Specificity: Found in a variety of tissues. Mostly abundant in brain and spine. Post-translational modifications: Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631. Similarity: Belongs to the kinesin light chain family. Contains 6 TPR repeats. SWISS: Q07866 Gene ID: 3831 Database links: Entrez Gene: 3831 Human Entrez Gene: 16593 Mouse Omim: 600025 Human SwissProt: Q07866 Human SwissProt: O88447 Mouse Unigene: 20107 Human Unigene: 657678 Human Unigene: 278357 Mouse Unigene: 106230 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human HeLa cell lysates
Primary: Anti-KLC1 (bs-11212R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
