| 产品编号 | bs-11346R |
| 英文名称 | EGFLAM |
| 中文名称 | 皮卡丘素抗体 |
| 别 名 | fibronectin type-III and laminin G-like domain-containing protein; Agrin-like protein; AGRINL; AGRNL; EGF-like; EGF-like, fibronectin type III and laminin G domains; EGF-like, fibronectin type-III and laminin G-like domain-containing protein; EGFLA_HUMAN; EGFLAM; FLJ39155; Pikachurin. |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞类型标志物 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 109kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pikachurin: 111-210/1017 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
EGFLAM is a 1,017 amino acid secreted protein containing three EGF-like domains, two fibronectin type-III domains, and three laminin G-like domains. Colocalizing with bassoon, CtBP and dystroglycan in photoreceptor synaptic terminals, EGFLAM is involved in retinal photoreceptor ribbon synapse formation. EGFLAM may also promote matrix assembly and cell adhesion. Existing as five alternatively spliced isoforms, the gene encoding EGFLAM maps to human chromosome 5p13.2. Chromosome 5 makes up approximately 6% of the human genome and contains 181 million base pairs, which encode 1,000 genes. Cockayne syndrome, Treacher Collins syndrome, acute myelogenous leukemias and myelodysplastic syndrome are associated with genes present on chromosome 5. Function: Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness. Subunit: Interacts with DAG1 alpha-dystroglycan Subcellular Location: Secreted; extracellular space; extracellular matrix. Cell junction > synapse. Detected in the synaptic cleft of the ribbon synapse around the postsynaptic terminals of bipolar cells. Colocalizes with BSN, CTBP2 and DAG1 in photoreceptor synaptic terminals. Post-translational modifications: O-glycosylated; contains chondroitin sulfate and heparan sulfate. Similarity: Contains 3 EGF-like domains. Contains 2 fibronectin type-III domains. Contains 3 laminin G-like domains. SWISS: Q63HQ2 Gene ID: 133584 Database links: Entrez Gene: 133584 Human Entrez Gene: 268780 Mouse SwissProt: Q63HQ2 Human SwissProt: Q4VBE4 Mouse Unigene: 20103 Human Unigene: 203208 Mouse Unigene: 27713 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
