| 产品编号 | bs-11402R |
| 英文名称 | GCX1/C20orf100 Rabbit pAb |
| 中文名称 | 20号染色体开放阅读框100抗体 |
| 别 名 | Chromosome 20 open reading frame 100; Chromosome 20 open reading frame 100; dJ1108D11.2; dJ495O3.1; GCX 1; GCX1; GCX-1; Granulosa cell HMG box protein 1; MGC15880; Weakly similar to NONHISTONE CHROMOSOMAL PROTEIN 6A [S.cerevisiae]; Weakly similar to NONHISTONE CHROMOSOMAL PROTEIN 6A [S.cerevisiae]; TOX2_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCX1/C20orf100: 201-300/488 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GCX-1 is a 488 amino acid transcription activator that is restricted to expression in the hypothalamus, pituitary, ovary, testis and uterus.
Human reproduction is controlled by the hypothalamic-pituitary gonadal axis (HPGA) that is laid down early in fetal development. This expression pattern in the HPGA suggests that this nuclear protein is likely related to specific events in reproduction, particularly in the female. GCX-1 contains a HMG-box domain, which is commonly found in proteins that function as intercellular regulators and transcriptional co-regulators, and are found to be involved in important events such as sex determination and in the regulation of T cell differentiation. There are two isoforms of GCX-1 which are produced as a result of alternative splicing events. Function: C20orf100 is a putative transcriptional activator involved in the hypothalamo-pituitary-gonadal system. It may regulate follicular development and other processes related to female reproduction. Subcellular Location: Nuclear Similarity: Contains 1 HMG box DNA-binding domain. SWISS: Q96NM4 Gene ID: 84969 Database links: Entrez Gene: 84969 Human Entrez Gene: 269389 Mouse Omim: 611163 Human SwissProt: Q96NM4 Human Unigene: 26608 Human Unigene: 129021 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCX1 C20orf100) Polyclonal Antibody, Unconjugated (bs-11402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCX1 C20orf100) Polyclonal Antibody, Unconjugated (bs-11402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
