| 产品编号 | bs-11444R |
| 英文名称 | Rabbit Anti-NOCT antibody |
| 中文名称 | 分子生物钟调控蛋白NOC抗体 |
| 别 名 | NOCT_HUMAN; Nocturnin; CCR4; CCRN4L; NOC; EC:3.1.3.108; Carbon catabolite repression 4-like protein; CCR4L; Ccr4c; |
| 研究领域 | 细胞生物 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nocturnin: 231-330/431 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nocturnin is a 431 amino acid circadian deadenylase protein that is expressed in a broad range of tissues with greatest abundance in the liver, kidney and testis. Nocturnin plays a role in circadian regulation as well as diet-induced obesity. The mRNA abundance of Nocturnin exhibits circadian rhythmicity, peaking after dusk in photoreceptors, spleen, heart, kidney and liver. Nocturnin is thought to be responisble for turning off genes that are involved in circadian regulation. In Xenopus retinal photoreceptor cells, the rhythmic regulation of Nocturnin is thought to be controlled by phosphorylated CREB. Mice lacking Nocturnin remain lean on high fat diets with a reduction in visceral fat, which suggests that this protein may also be responsible for lipid metabolism and fat storage. Function: CCRN4L is highly similar to Nocturnin, a gene identified as a circadian clock regulated gene in Xenopus laevis. This protein and Nocturnin protein share similarity with the C terminal domain of a yeast transcription factor, carbon catabolite repression 4 (CCR4). The mRNA abundance of a similar gene in mouse has been shown to exhibit circadian rhythmicity, which suggests a role for this protein in clock function or as a circadian clock effector. Similarity: Belongs to the CCR4/nocturin family. SWISS: Q9UK39 Gene ID: 25819 Database links: Entrez Gene: 25819 Human Omim: 608468 Human SwissProt: Q9UK39 Human Unigene: 480763 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebellum tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Cerebellum tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Human A431 cell lysates Lane 6: Human HL-60 cell lysates Primary: Anti-Nocturnin (bs-11444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 53 kDa 
Sample:
Kidney (Mouse) Lysate at 40 ug Primary: Anti-Nocturnin (bs-11444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
