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Rabbit Anti-Gli2  antibody (bs-11564R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-11564R
英文名称 Gli2
中文名称 转录因子Gli2抗体
别    名 Gli 2; Gli-2; GLI family zinc finger 2; GLI kruppel family member 2; GLI Kruppel family member GLI2; GLI2; GLI2_HUMAN; Oncogene GLI2; Tax helper protein 2; Tax helper protein; Tax responsive element 2 holding protein; Tax responsive element 25 bp sequence binding protein; THP 2; THP; THP2; Zinc finger protein GLI2.  
Specific References  (3)     |     bs-11564R has been referenced in 3 publications.
[IF=11.556] Chao Tang. et al. Hedgehog signaling is controlled by Rac1 activity. Theranostics. 2022; 12(3): 1303–1320  WB,IP ;  Mouse.  
[IF=3.73] Zhang, Qiang, et al. "Serotonin Receptor 2C and Insulin Secretion." PloS one 8.1 (2013): e54250.  WB ;  Mouse.  
[IF=2.705] Wu J et al. Nicotine inhibits murine Leydig cell differentiation and maturation via regulating Hedgehog signal pathway. Biochem Biophys Res Commun. 2019 Feb 26;510(1):1-7.  WB ;  Mouse.  
研究领域 肿瘤  神经生物学  信号转导  干细胞  转录调节因子  锌指蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Pig,Dog)
产品应用 IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:25, IF=1:100-500, Flow-Cyt=3ug/test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 168kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Gli2: 501-600/1586 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone.

Function:
FunctionActs as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.

Subunit:
Interaction with ZIC

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated in vitro by ULK3.

DISEASE:
Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.

Similarity:
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.

SWISS:
P10070

Gene ID:
2736

Database links:

UniProtKB/Swiss-Prot: P10070.4

Entrez Gene: 2736 Human

Entrez Gene: 14633 Mouse

Omim: 165230 Human

SwissProt: P10070 Human

SwissProt: Q0VGT2 Mouse

SwissProt: Q8K0K3 Mouse

Unigene: 111867 Human

Unigene: 273292 Mouse



产品图片
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Gli2 Rabbit pAb) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Gli2 Rabbit pAb) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gli2) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Gli2) polyclonal Antibody, Unconjugated (bs-11564R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Gli2 antibody (bs-11564R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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