| 产品编号 | bs-10057R |
| 英文名称 | PCV2 capsid protein |
| 中文名称 | 猪圆环病毒Ⅱ型衣壳蛋白抗体 |
| 别 名 | ORF1; ORF2 (PCV-2; PCV 2; Porcine circovirus2; Porcine circovirus-2.) |
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Specific References (1) | bs-10057R has been referenced in 1 publications.
[IF=4.32] Liu et al. Cubosome nanoparticles potentiate immune properties of immunostimulants. (2016) Int.J.Nanomedicin. 11:3571-83 IHC-P ; Mouse.
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| 研究领域 | 细胞生物 免疫学 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | PCV2 |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from PCV2 capsid protein: 133-233/233 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
There has been much uncertainty in the relationship between PCV2 and PMWS but Bolin et al demonstrated that Koch's postulates could be fulfilled by inoculating cesarean-derived, colostrum-deprived (CDCD) pigs with PCV2 and producing clinical signs of PMWS. Similar work has been performed using an infectious genomic clone of PCV2 in specific pathogen free (SPF) pigs to produce the gross and microscopic lymphoid lesions of PMWS. Although PCV2 has been shown to induce PMWS lesions in CDCD and SPF pigs, the presence of additional factors are required. Function: Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope. Subunit: Homomultimer. Assembles in the nucleus, presumably in an immature form, then migrates to the cytoplasm once assembled as mature virion (Probable). Interacts with Rep; this interaction relocates Rep into the nucleus. Subcellular Location: Host nucleus. Virion (Potential). Similarity: Belongs to the circoviridae capsid protein family. SWISS: O56129 Gene ID: 2943185 Database links: Entrez Gene: 2943185 PCV2 SwissProt: O56129 PCV2 猪圆环病毒-PCV-2是一种嗜单核-巨噬细胞性的病毒，属于圆环病毒科圆环病毒属的成员，根据抗原性及基因组成，分为PCVl和PCV2两种，其中PCV2对猪具有致病性，PCV2(病毒囊膜衣壳蛋白(Cap))主要侵害免疫系统，导致感染猪免疫抑制，降低机体对其他病原体的抵抗力和疫苗的反应性，容易导致其他病毒和细菌的继发感染，使死亡率明显升高。 |
| 产品图片 |
Sample:
Lane 1: Recombinant PCV2 Cap protein, Trx & His(bs-41401P-500ng) Primary: Anti-PCV2 capsid protein (bs-10057R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 44 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
