产品中心-北京博奥森生物技术有限公司

产品编号	bs-11764R
英文名称	OPA1
中文名称	视神经萎缩相关蛋白1抗体
别名	Dynamin like 120 kDa protein; Dynamin like 120 kDa protein, mitochondrial; Dynamin-like 120 kDa protein; Dynamin-like 120 kDa protein, form S1; FLJ12460; Juvenile kjer type optic atrophy; Juvenile kjer-type optic atrophy; KIAA0567; KJER type; Large GTP binding protein; largeG; MGM1; Mitochondrial dynamin like 120 kDa protein; Mitochondrial dynamin like GTPase; NPG; NTG; OAK; OPA 1; OPA1; OPA1 gene; OPA1_HUMAN; Optic atrophy 1 (autosomal dominant); OPTIC ATROPHY 1; Optic atrophy 1 gene protein; Optic atrophy 1 homolog (human); Optic atrophy protein 1; Optic atrophy protein 1 homolog.
	Specific References (13) \| bs-11764R has been referenced in 13 publications. [IF=13.352] Guangdong Bai. et al. Perinatal exposure to glyphosate-based herbicides impairs progeny health and placental angiogenesis by disturbing mitochondrial function. ENVIRON INT. 2022 Dec;170:107579 WB ; Pig. PubMed:36265358 [IF=6.792] Yang Q et al. Hexavalent chromium induces mitochondrial dynamics disorder in rat liver by inhibiting AMPK/PGC-1α signaling pathway. Environ Pollut.2020 Oct;265(Pt A):114855. WB ; Rat. PubMed:32474337 [IF=6.513] Zhe Li. et al. Bisphenol A aggravate selenium deficiency-induced apoptosis via miR-215-3p/Dio1 to activate ROS/PI3K/AKT pathway in chicken arterial. J CELL PHYSIOL. 2023 Apr;: WB ; Chicken. PubMed:37012668 [IF=5.895] Xixi Wang. et al. Cannabidiol Alleviates Perfluorooctanesulfonic Acid-Induced Cardiomyocyte Apoptosis by Maintaining Mitochondrial Dynamic Balance and Energy Metabolic Homeostasis. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXX WB ; Mouse,Rat. PubMed:37010249 [IF=5.396] Yi Zhao. et al. Role of mitochondria-endoplasmic reticulum coupling in lycopene preventing DEHP-induced hepatotoxicity. Food Funct. 2021 Sep;: WB ; mouse. PubMed:34608470 [IF=4.527] Wang S et al. Hydrogen sulfide-induced oxidative stress leads to excessive mitochondrial fission to activate apoptosis in broiler myocardia. Ecotoxicol Environ Saf. 2019 Aug 20;183:109578. WB ; broiler. PubMed:31442807 [IF=4.427] Wang Y et al.Fine particulate matter induces mitochondrial dysfunction and oxidative stress in human SH-SY5Y cells.(2018) Chemosphere;218:577-588. WB ; PubMed:30502696 [IF=3.708] Xuena Zhang. et al. Luteoloside Prevents Sevoflurane-induced Cognitive Dysfunction in Aged Rats via Maintaining Mitochondrial Function and Dynamics in Hippocampal Neurons. NEUROSCIENCE. 2023 Feb;: WB ; Rat. PubMed:36764603 [IF=3.7] Ku, Tingting, et al. "PM 2.5, SO 2 and NO 2 co-exposure impairs neurobehavior and induces mitochondrial injuries in the mouse brain."Chemosphere 163 (2016): 27-34. WB ; Mouse. PubMed:27521637 [IF=2.835] Yuan Zhang a et al. Electroacupuncture ameliorates endotoxin-induced acute lung injury in rabbits by regulating heme oxygenase-1 expression to improve mitochondrial dynamics. Journal of King Saud University – Science, 2019. WB ; Rabbit. PubMed:DOI:10.1016/j.jksus.2019.11.029 [IF=2.752] Yuliang Wen. et al. Changes in the Mitochondrial Dynamics and Functions Together with the mRNA/miRNA Network in the Heart Tissue Contribute to Hypoxia Adaptation in Tibetan Sheep. Animals-Basel. 2022 Jan;12(5):583 IHC ; Sheep. PubMed:35268153 [IF=2.323] Guan Wang. et al. Effects of Aging on Expression of Mic60 and OPA1 and Mitochondrial Morphology in Myocardium of Tibetan Sheep. Animals-Basel. 2020 Nov;10(11):2160 IHC ; Sheep. PubMed:33233488 [IF=1.35] Hui, Yan, et al. "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." Acta Histochemica (2017). WB ; Rat. PubMed:28434671
研究领域	心血管细胞生物神经生物学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Rat (predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	111kDa
细胞定位	细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human OPA1: 651-750/960
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	OPA1 is a 120kDa protein belonging to the dynamin family. The OPA1 gene has been localized to 3q29. The gene is targeted to mitochondria and is involved in mitochondrial biogenesis. Defects in OPA1 are a cause of optic atrophy type 1. OPA1 is mostly expressed in retina but can also be expressed in brain, testis, heart and skeletal muscle. Function: Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Subcellular Location: Mitochondrion inner membrane. Mitochondrion intermembrane space. Tissue Specificity: Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. Post-translational modifications: PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. DISEASE: Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness. Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. Similarity: Belongs to the dynamin family. SWISS: O60313 Gene ID: 4976 Database links: Entrez Gene: 424900 Chicken Entrez Gene: 4976 Human Entrez Gene: 74143 Mouse Entrez Gene: 171116 Rat Omim: 605290 Human SwissProt: O60313 Human SwissProt: P58281 Mouse SwissProt: Q2TA68 Rat Unigene: 594504 Human Unigene: 274285 Mouse Unigene: 9783 Rat
产品图片	Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-OPA1 (bs-11764R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OPA1 Polyclonal Antibody, Unconjugated(bs-11764R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记