| 产品编号 | bs-11819R |
| 英文名称 | CHRDL1 Rabbit pAb |
| 中文名称 | 脊索生成素样蛋白1抗体 |
| 别 名 | CHL; chordin like 1; Chordin-like protein 1; CHRDL1; CRDL1_HUMAN; dA141H5.1; neuralin 1; Neuralin-1; Neurogenesin-1; NRLN1; Ventroptin; VOPT. |
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Specific References (1) | bs-11819R has been referenced in 1 publications.
[IF=0] Bhutani, Nidhi, et al. "USE OF CHORDIN-LIKE 1 OR COLLAGEN VI WITH MESENCHYMAL STEM CELLS." U.S. Patent No. 20,170,209,621. 27 Jul. 2017. Human.
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| 研究领域 | 细胞生物 发育生物学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHRDL1: 21-120/450 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The patterning of the central nervous system (CNS) relies on the interaction of multiple signaling molecules, including Shh (sonic hedgehog homolog), Wnt proteins and BMPs (bone morphogenetic proteins), with their antagonists, namely chordin and Noggin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like BMPs and sequestering them in latent complexes. CHRDL1 (Chordin-like protein 1), also known as CHL, VOPT (Ventroptin) or NRLN1 (Neuralin-1), is a 450 amino acid secreted protein that contains three VWFC domains. Functioning in a similar manner to chordin, CHRDL1 binds to BMP-4 and prevents the interaction of BMP-4 with its target receptors, thereby antagonizing BMP-4 activity. Additionally, CHRDL1 is thought to play a role in dorsoventral axis formation, embryonic bone formation and angiogenesis, as well as in the differentiation of neural stem cells. Function: Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embyonic bone formation (By similarity). May also play an important role in regulating retinal angiogenesis trough modulation of BMP4 actions in endothelial cells. Subcellular Location: Secreted. Tissue Specificity: Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes. Similarity: Contains 3 VWFC domains. SWISS: Q9BU40 Gene ID: 91851 Database links: Entrez Gene: 91851 Human Entrez Gene: 83453 Mouse Omim: 300350 Human SwissProt: Q9BU40 Human SwissProt: Q920C1 Mouse Unigene: 496587 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Eye tissue lysates
Lane 2: Mouse Prostate tissue lysates
Primary: Anti-CHRDL1 (bs-11819R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 52 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
