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Rabbit Anti-Jarid2  antibody (bs-11860R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-11860R
英文名称 Jarid2
中文名称 组蛋白去甲基化酶JARID2抗体
别    名 Jumonji; JARD2; JARD2_HUMAN; JARID2; JMJ; Jumonji AT rich interactive domain 2; Jumonji protein ; Jumonji/ARID domain containing protein 2; Jumonji/ARID domain-containing protein 2 ; Protein Jumonji.  
Specific References  (2)     |     bs-11860R has been referenced in 2 publications.
[IF=2.089] Yiran Gu. et al. JARID2 regulates epithelial mesenchymal transition through the PTEN/AKT signalling pathways in ovarian endometriosis. REPROD BIOL. 2023 Mar;23:100729  IHC,WB ;  Human.  
[IF=1.768] Yoshinobu Ichikawa. et al. Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica). J POULT SCI. 2023 Jan;60(1):2023001  WB ;  Coturnix japonica.  
研究领域 肿瘤  细胞生物  神经生物学  信号转导  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 139kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Jarid2/Jumonji: 301-400/1246 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012]

Function:
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.

Subunit:
Associates with a histone methyltransferase complex containing GLP1/EHMT1 and G9a/EHMT2. Interacts with SUZ12; the interaction is direct. Interacts with GATA4 (via the N-terminal region). Interacts with NKX2-5 (via the C-terminal region). Interacts with RB1. Interacts with ZNF496 (By similarity). Associates with the PRC2 complex, which includes EED, EZH1, EZH2, SUZ12, RBBP4 and AEBP2; JARID2 is probably not a core component of the PRC2 complex and associates to PRC2 via its interaction with SUZ12.

Subcellular Location:
Nucleus. Colocalizes with the PRC2 complex on chromatin.

Tissue Specificity:
During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.

Similarity:
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.

SWISS:
Q92833

Gene ID:
3720

Database links:

Entrez Gene: 3720 Human

Entrez Gene: 16468 Mouse

Omim: 601594 Human

SwissProt: Q92833 Human

SwissProt: Q62315 Mouse

Unigene: 269059 Human

Unigene: 25059 Mouse



产品图片
Sample:
Molt-4(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- Jarid2 (bs-11860R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 139 kD
Observed band size: 139 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Jarid2/Jumonji Polyclonal Antibody, Unconjugated(bs-11860R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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