| 产品编号 | bs-11879R |
| 英文名称 | LGI3 Rabbit pAb |
| 中文名称 | Lgi3蛋白抗体 |
| 别 名 | Leucine rich repeat LGI family member 3; Leucine-rich glioma inactivated protein 3; Leucine-rich glioma-inactivated protein 3; Leucine-rich repeat LGI family member 3; LGI1 like protein 4; LGI1-like protein 4; LGI3; LGI3_HUMAN; LGIL4. |
| 研究领域 | 细胞生物 发育生物学 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 59 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LGI3: 101-220/548 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic å/∫ horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. Leucine-rich glioma-inactivated protein 3 (LGI3), also known as LGI1-like protein 4 (LGIL4) or leucine-rich repeat LGI family member 3, is a 548 amino acid secretory protein. LGI3 contains five LRR repeats and seven EAR repeats. Widely expressed, with highest levels in brain and lung, LGI3 has been shown to colocalize with endocytosis-associated proteins, lipid raft markers, and Syntaxin. The gene encoding LGI3 maps to chromosome 8p21.3. Function: May participate in the regulation of neuronal exocytosis. Subunit: Interacts with STX1A Subcellular Location: Secreted (Potential). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle (By similarity). Cell junction, synapse, synaptosome (By similarity). Note=Found in the synaptosomal membrane fraction Tissue Specificity: Widely expressed, with highest levels in brain and lung. Similarity: Contains 7 EAR repeats. Contains 3 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q8N145 Gene ID: 203190 Database links: Entrez Gene: 203190 Human Entrez Gene: 213469 Mouse Omim: 608302 Human SwissProt: Q8N145 Human SwissProt: Q8K406 Mouse |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- LGI3 (bs-11879R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LGI3) Polyclonal Antibody, Unconjugated (bs-11879R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
