扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-ADGRE1  antibody (bs-7058R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-7058R
英文名称 ADGRE1
中文名称 表皮生长因子样激素受体1(EMR1)抗体
别    名 F4/80; Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; AGRE1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3.  
Specific References  (10)     |     bs-7058R has been referenced in 10 publications.
[IF=9.918] Daijun Zhou. et al. An injectable miR181a-IFI6 nanoparticles promote high-quality healing of radiation-induced skin injury. MATER TODAY ADV. 2022 Aug;15:100267  FC ;  Human.  
[IF=7.675] Tao Yang. et al. Sphingosine-1-Phosphate Alleviates Irradiation Induced Salivary Gland Hypofunction through Preserving Endothelial Cells and Resident Macrophages. ANTIOXIDANTS-BASEL. 2022 Oct;11(10):2050  IF ;  Mouse.  
[IF=6.93] Kwan, Karen HL, et al. ʺSilver nanaoparticles alter proteoglycan expression in the promotion of tendon repair.ʺ Nanomedicine: Nanotechnology, Biology and Medicine (2013).  IHC-P ;  Rat.  
[IF=5.923] Liang Hu. et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci. 2021 Jan;22(24):13493  WB ;  Pig.  
[IF=5.714] Ruyi Qu. et al. Glucocorticoids improve the balance of M1/M2 macrophage polarization in experimental autoimmune uveitis through the P38MAPK-MEF2C axis. INT IMMUNOPHARMACOL. 2023 Jul;120:110392  FCM ;  Rat.  
[IF=4.396] Yosuke Asami. et al. Rat peri-implant soft tissue specifically expressed CXCL2 on titanium implant during wound healing. 2021 Nov 30  IHC ;  Rat.  
[IF=3.36] Tsang, Yuk-Wah, et al. "Improving immunological tumor microenvironment using electro-hyperthermia followed by dendritic cell immunotherapy." BMC Cancer 15.1 (2015): 708.  IHC-P ;  Mouse.  
[IF=2.751] Jiachen Lv. et al. Alcohol Aggravates Acute Pancreatitis by Impairing Autophagic Flux Through Activation of AMPK Signaling Pathway. 2021 Feb 08  IHC ;  Human,Rat.  
[IF=2.435] AHMED M et al. Molecular Imaging of a New Multimodal Microbubble for Adhesion Molecule Targeting. BIOMEDICAL ENGINEERING SOCIETY.2018 Nov.  FCM ;  Mouse&Human.  
[IF=1.632] Liu Y et al. Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood.(2018)Vet Immunol Immunopathol. Nov;205:83-92.  IF ;  Sheep.  
研究领域 免疫学  生长因子和激素  G蛋白偶联受体  糖蛋白  G蛋白信号  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat,Pig,GuineaPig)
产品应用 WB=1:500-2000, Flow-Cyt=2μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 95kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ADGRE1: 701-800/886 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function:
Could be involved in cell-cell interactions.

Subunit:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Wide expression; increased levels in peripheral blood mononuclear cells.

Similarity:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains.
Contains 1 GPS domain.

SWISS:
Q14246

Gene ID:
2015

Database links:

Entrez Gene: 2015 Human

Entrez Gene: 13733 Mouse

Omim: 600493 Human

SwissProt: Q14246 Human

SwissProt: Q61549 Mouse

Unigene: 2375 Human

Unigene: 2254 Mouse




产品图片
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-EMR1 (bs-7058R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- EMR1 (bs-7058R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- EMR1 (bs-7058R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-EMR1 antibody (bs-7058R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Mouse brain.
Primary Antibody (green line): Rabbit Anti-EMR1 antibody (bs-7058R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Positive control: mouse Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody : rabbit Anti-EMR1 bs-7058R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA.
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (bs-7058R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版权所有 2004-2026 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    认证编号: 00122Q31509R1M/1100
京ICP备05066980号-1         京公网安备110107000727号