| 产品编号 | bs-11986R |
| 英文名称 | CABP1 Rabbit pAb |
| 中文名称 | 钙结合蛋白1抗体 |
| 别 名 | CABP 1; CABP1; CABP-1; CABP1_HUMAN; Calbrain; Calcium binding protein 1; Calcium binding protein 5; Calcium-binding protein 1; Caldendrin; HCALB BR; HCALB_BR. |
| 研究领域 | 细胞生物 神经生物学 信号转导 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CABP1: 311-355/370 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Calcium plays an essential role in many biological processes. The calcium binding protein (CaBP) family shares much similarity to calmodulin. It has been shown that CaBP proteins can substitute functionally for, and probably augment the function of, calmodulin. Calcium binding proteins are a crucial part of calcium mediated cellular signal transduction in the central nervous system. There are several members of the family with varying expression patterns. CaBP1 and CaBP2 can be expressed as multiple, alternatively spliced variants in brain and retina. CaBP3, CaBP4 and CaBP 5 are restricted to retinal rod and cone cells. CaBP7 shares 70% homology with CaBP8 and 50% homology with CaM I. Function: Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D (By similarity). Inhibits TRPC5 channels. Prevents NMDA receptor-induced cellular degeneration. Subunit: Homodimer; when bound to calcium or magnesium. Interacts (via C-terminus) with ITPR1, ITPR2 and ITPR3. This binding is calcium dependent and the interaction correlates with calcium concentration. An additional calcium-independent interaction with the N-terminus of ITPR1 results in a decreased InsP(3) binding to the receptor. Interacts with CACNA1A (via C-terminal CDB motif) in the pre- and postsynaptic membranes. Interacts with CACNA1C (via C-terminal C and IQ motifs). The binding to the C motif is calcium independent whereas the binding to IQ requires the presence of calcium and is mutually exclusive with calmodulin binding. Interacts with CACNA1D (By similarity). Interacts with TRPC5 (via C-terminus). Interacts (via EF-hands 1 and 2) at microtubules with MAP1LC3B. Interacts with MYO1C and NELF Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane. Tissue Specificity: Retina and brain. Somatodendritic compartment of neurons. Calbrain was found exclusively in brain where it is abundant in the hippocampus, habenular area in the epithalamus and in the cerebellum. Post-translational modifications: Phosphorylated. The phosphorylation regulates the activity. Similarity: Contains 4 EF-hand domains. SWISS: Q9NZU7 Gene ID: 9478 Database links: Entrez Gene: 9478 Human Entrez Gene: 29867 Mouse Omim: 605563 Human SwissProt: Q9NZU7 Human SwissProt: Q9JLK7 Mouse Unigene: 458482 Human Unigene: 38551 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Kidney tissue lysates
Lane 5: Rat Pancreas tissue lysates
Lane 6: Human Jurkat cell lysates
Primary: Anti-CABP1 (bs-11986R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 26 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CABP1) Polyclonal Antibody, Unconjugated (bs-11986R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
