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Rabbit Anti-GABRA2  antibody (bs-12061R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-12061R
英文名称 GABRA2
中文名称 G氨基丁酸A型受体α2/GABAA Rα2抗体
别    名 GABA A Receptor a2; GABA A Receptor alpha 2; GABA A receptor subunit alpha 2; GABA; GABA(A) receptor subunit alpha 2; GABA(A) receptor subunit alpha-2; GABR A2; GABRA 2; GABRA2; GABRA2 protein; Gamma aminobutyric acid (GABA) A receptor alpha 2; Gamma aminobutyric acid A receptor alpha 2; Gamma aminobutyric acid receptor alpha 2 subunit; Gamma aminobutyric acid receptor subunit alpha 2; Gamma-aminobutyric acid receptor subunit alpha-2; GBRA2_HUMAN.  
Specific References  (2)     |     bs-12061R has been referenced in 2 publications.
[IF=6.208] Yaiza M. Arenas. et al. Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia. INT J MOL SCI. 2022 Jan;23(19):11770  WB ;  Rat.  
[IF=5.59] Sancho-Alonso, María. et al. Hyperammonemia Enhances GABAergic Neurotransmission in Hippocampus: Underlying Mechanisms and Modulation by Extracellular cGMP. Mol Neurobiol. 2022 Mar;:1-18  WB ;  Rat.  
研究领域 神经生物学  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse,Rat (predicted: Human,Dog,Pig,Cow,Horse,Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 48kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GABRA2: 185-280/451 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit:
Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular Location:
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.

SWISS:
P47869

Gene ID:
2555

Database links:

Entrez Gene: 2555 Human

Entrez Gene: 14395 Mouse

Entrez Gene: 289606 Rat

Entrez Gene: 282236 Cow

Omim: 137140 Human

SwissProt: P10063 Cow

SwissProt: P47869 Human

SwissProt: P26048 Mouse

SwissProt: P23576 Rat

Unigene: 116250 Human

Unigene: 5304 Mouse

Unigene: 48180 Rat



产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GABRA2 (bs-12061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Cerebral cortex(Rat) Lysate at 40 ug
Primary: Anti-GABRA2 (bs-12061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample: Cerebrum (mouse) Lysate at 40 ug
Primary: Anti- GABRA2(bs-12061R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated(bs-12061R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- GABRA2 antibody(bs-12061R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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