| 产品编号 | bs-7166R |
| 英文名称 | MUC5AC |
| 中文名称 | 胃粘液素抗体 |
| 别 名 | MUC5AC Glycoprotein; Gastric Mucin M1; Apomucin; Major Airway Glycoprotein; MUC 5; MUC5; Mucin 5 subtype AC tracheobronchial; Mucin 5 Subtypes A And C; Mucin 5 subtypes A and C tracheobronchial/gastric; TBM; Tracheobronchial Mucin. |
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Specific References (5) | bs-7166R has been referenced in 5 publications.
[IF=8.46] Taki, K., et al. "GNASR201H and KrasG12D cooperate to promote murine pancreatic tumorigenesis recapitulating human intraductal papillary mucinous neoplasm." Oncogene 35.18 (2016): 2407-2412. IHC-P ; Mouse.
[IF=7.076] Annika Sünderhauf. et al. Loss of Mucosal p32/gC1qR/HABP1 Triggers Energy Deficiency and Impairs Goblet Cell Differentiation in Ulcerative Colitis. Cell Mol Gastroenter. 2021 Jan;: ELISA,IHC ; Mouse.
[IF=5.923] Mohamed Elbadawy. et al. Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. Int J Mol Sci. 2021 Jan;22(19):10763 IHC ; Bat.
[IF=3.317] Tomchaney, M.. et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease. Bmc Pulm Med. 2021 Dec;21(1):1-14 IF ; Human, Mouse.
[IF=2.121] Sasagu Kimura. et al. Histological characteristics of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in asthmatic murine model during A(H1N1)pdm09 infection. PATHOL INT. 2022 Sep;: IHC ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Horse) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 523kDa |
| 细胞定位 | 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MUC5AC/Mucin 5AC: 601-700/5030 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mucin is a high M.W. (>1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in Müller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus). The expression of MUC5AC has been frequently observed in gastric carcinomas and may be used as a marker of gastric differentiation. Function: Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. Subunit: Multimeric. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD). Subcellular Location: Secreted; Cytoplasmic and cell surface Tissue Specificity: Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. Post-translational modifications: C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. Similarity: Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 2 VWFC domains. Contains 4 VWFD domains. SWISS: P98088 Gene ID: 4586 Database links: Entrez Gene: 4586 Human Entrez Gene: 17833 Mouse Omim: 158373 Human SwissProt: P98088 Human Unigene: 534332 Human Unigene: 10789 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (bs-7166R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (bs-7166R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (bs-7166R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
