| 产品编号 | bs-12246R |
| 英文名称 | Pumilio 2 |
| 中文名称 | PUM2蛋白抗体 |
| 别 名 | FLJ36528; KIAA0235; MGC138251; MGC138253; PUM 2; Pum2; PUM2; PUMH2; Pumilio (Drosphila) homolog 2; Pumilio homolog 2 (Drosophila); Pumilio homolog 2; Pumilio2; PUM2_HUMAN. |
| 研究领域 | 发育生物学 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 114kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human Pumilio 2: 901-1066/1066 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pumilio 2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding mRNA targets. It supports proliferation and self-renewal of stem cells by regulating the translation of key transcripts. The Pumilio gene encodes proteins that are required for development of germ stem cells in one or both sexes. The Pumilio protein interacts with the human Nanos1 protein and this interaction may play a conserved role in germ cell development. Pumilio 2 is highly expressed in testis and ovary and at lower levels in brain, heart, kidney, liver, muscle, placenta, intestine and stomach. It is also expressed in stem cells, germ cells and in most fetal tissues. Function: Pumilio 2, a human homolog of Pumilio, a protein required to maintain germ line stem cells in Drosophila and Caenorhabditis elegans, forms a stable complex with DAZ through the same functional domain required for RNA binding, protein-protein interactions and rescue of Pumilio mutations in flies. Pumilio 2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells. Pumilio 2 is a component of conserved cellular machinery that may be required for germ cell development. Subunit: Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Binds to a RNA consensus sequence, that is related to the Nanos Response Element (NRE), a 16 bp sequence found in the 3'-UTR of the Drosophila hb mRNA. Also binds to the NRE. Interacts with NANOS3 (By similarity). Interacts with SNAPIN. Subcellular Location: Cytoplasm (Probable). Cytoplasmic granule (By similarity). Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules (By similarity). Co-localizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. Tissue Specificity: Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. Similarity: Contains 1 PUM-HD domain. Contains 8 pumilio repeats. SWISS: Q8TB72 Gene ID: 23369 Database links: Entrez Gene: 23369 Human Entrez Gene: 80913 Mouse Omim: 607205 Human SwissProt: Q8TB72 Human SwissProt: Q80U58 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
