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Rabbit Anti-DNA polymerase delta  antibody (bs-7416R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-7416R
英文名称 DNA polymerase delta
中文名称 DNA聚合酶δ 抗体
别    名 DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; DNA polymerase subunit delta 2; DNA polymerase subunit delta p50; DPOD2_HUMAN; POLD 2; POLD2.  
研究领域 细胞生物  染色质和核信号  信号转导  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse,Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 51kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Human DNA polymerase delta: 101-200/469 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). These exonucleases include the family of DNA polymerases (3). DNA pol α, β, ∂, and e are involved in DNA replication and repair (4). DNA pol ∂ and DNA pol e are multisubunit enzymes, with DNA pol ∂ consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50 (5). The nuclear-encoded DNA pol © is the only DNA polymerase required for the replication of the mitochondrial DNA (6). DNA pol Ω is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis (7). DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks (8).

Function:
The function of the small subunit is not yet clear.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the DNA polymerase delta/II small subunit family.

SWISS:
P49005

Gene ID:
5425

Database links:

Entrez Gene: 281991 Cow

Entrez Gene: 5425 Human

Entrez Gene: 18972 Mouse

Entrez Gene: 289758 Rat

Omim: 600815 Human

SwissProt: P49004 Cow

SwissProt: P49005 Human

SwissProt: O35654 Mouse

SwissProt: Q6AXY4 Rat

Unigene: 306791 Human

Unigene: 35788 Mouse

Unigene: 22989 Rat



产品图片
Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-DNA polymerase delta (bs-7416R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-DNA polymerase delta (bs-7416R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POLD2) Polyclonal Antibody, Unconjugated (bs-7416R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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