产品编号 | bs-12316R |
英文名称 | HHIP |
中文名称 | HHIP蛋白抗体 |
别 名 | FLJ20992; FLJ90230; Hedgehog interacting protein; Hedgehog-interacting protein; hedgehoginteracting protein; HHIP; HHIP_HUMAN; HIP. |
研究领域 | 细胞生物 发育生物学 干细胞 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Dog,Cow,Horse,Sheep) |
产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 77kDa |
细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HHIP: 501-600/700 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Hedgehog (Hh) signaling proteins are critical for growth and tissue patterning during development. Patched (Ptc), a putative 12 transmembrane receptor, binds to Sonic hedgehog and is suspected to be a negative regulator of Hh signaling. A family member of patched, designated patched 2, has been found to be co-expressed with Sonic hedgehog. Smoothened (Smo), a seven transmembrane receptor, is complexed with patched in many tissues and is believed to be an essential component in the Hh signaling pathway. Hhip (hedgehog-interacting protein) is able to bind to and may be a transcriptional target of all Hh proteins. Binding of Hhip to Hh proteins attenuates hedgehog signaling. Function: Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. Subunit: Interacts with all three hedgehog family members, SHH, IHH and DHH. Subcellular Location: Cytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment. Tissue Specificity: Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney. Similarity: Belongs to the HHIP family. Contains 2 EGF-like domains. SWISS: Q96QV1 Gene ID: 64399 Database links: Entrez Gene: 64399 Human Entrez Gene: 15245 Mouse Omim: 606178 Human SwissProt: Q96QV1 Human SwissProt: Q7TN16 Mouse Unigene: 507991 Human Unigene: 254493 Mouse |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |