| 产品编号 | bs-12325R |
| 英文名称 | PTCHD3 |
| 中文名称 | 修补相关蛋白PTCHD3抗体 |
| 别 名 | Patched domain containing 3; Patched domain-containing protein 3; Patched-related protein; Ptchd3; PTHD3_HUMAN; PTR. |
| 研究领域 | 细胞生物 发育生物学 信号转导 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 87kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human PTCHD3: 151-250/767 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PTCHD3 is a 767 amino acid multi-pass membrane protein that localizes to the midpiece of the sperm tail, where it is implicated in sperm function and development. A member of the patched family, PTCHD3 contains one SSD (sterol-sensing) domain and is encoded by a gene that maps to human chromosome 10p12.1. Spanning nearly 135 million base pairs, chromosome 10 makes up approximately 4.5% of total DNA in cells and encodes nearly 1,200 genes. Several protein-coding genes, including those that encode for chemokines, cadherins, excision repair proteins, early growth response factors (Egrs) and fibroblast growth receptors (FGFRs), are located on chromosome 10. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. Function: May play a role in sperm development or sperm function. Subcellular Location: Membrane. Localizes to the midpiece of the sperm tail. Tissue Specificity: Expressed in germ cells of the testis (at protein level). Similarity: Belongs to the patched family. Contains 1 SSD (sterol-sensing) domain. SWISS: Q3KNS1 Gene ID: 374308 Database links: Entrez Gene: 374308 Human Omim: 611791 Human SwissProt: Q3KNS1 Human Unigene: 631832 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
