| 产品编号 | bs-12365R |
| 英文名称 | SIX2 |
| 中文名称 | 转录因子同源框蛋白SIX2抗体 |
| 别 名 | Homeobox protein SIX2; OTTHUMP00000201649; Sine oculis homeobox (Drosophila) homolog 2; Sine oculis homeobox homolog 2; Sine oculis homeobox homolog 2 (Drosophila); SIX homeobox 2; Six2; SIX2_HUMAN. |
| 研究领域 | 细胞生物 发育生物学 干细胞 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SIX2: 101-200/291 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Six proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six2, Six4 (AREC3) and Six5 bind to the same DNA sequence, indicating that they may regulate the same target genes. Six1 and Six4 are both capable of transactivating MEF3 site containing reporter genes, such as myogenin. It has been demonstrated that alterations to homeobox-containing genes may result in cancer. Six1 expression has been shown to be absent or low in normal adult tissues, although it is expressed in several tumor types, including breast carcinoma. Six1 overexpression has been shown to abrogate the G2 cell cycle checkpoint. Six2 is highly expressed in fetal tissues but expression is limited in adult tissues. Function: May be involved in limb tendon and ligament development. Subcellular Location: Nucleus. Tissue Specificity: Strongly expressed in skeletal muscle. Similarity: Belongs to the SIX/Sine oculis homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q9NPC8 Gene ID: 10736 Database links: Entrez Gene: 10736 Human Entrez Gene: 20472 Mouse Omim: 604994 Human SwissProt: Q9NPC8 Human SwissProt: Q62232 Mouse Unigene: 101937 Human Unigene: 5039 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
