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Rabbit Anti-Caseine Kinase 1 alpha antibody (bs-12427R)
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说明书: 100ul  200ul
100ul/1580.00元
200ul/2480.00元
大包装/询价

产品编号 bs-12427R
英文名称 Caseine Kinase 1 alpha
中文名称 酪蛋白激酶casein kinase Iα抗体
别    名 Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975.   
研究领域 肿瘤  细胞生物  信号转导  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CK1A/Caseine Kinase 1 alpha:221-337/337 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Function:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Subunit:
Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Note=Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.

Similarity:
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.
Casein kinase I subfamily.
Contains 1 protein kinase domain.

SWISS:
P48729

Gene ID:
1452

Database links:

Entrez Gene: 1452 Human

Entrez Gene: 93687 Mouse

Entrez Gene: 113927 Rat

Omim: 600505 Human

SwissProt: P48729 Human

SwissProt: Q8BK63 Mouse

SwissProt: P97633 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caseine Kinase 1 alpha Polyclonal Antibody, Unconjugated(bs-12427R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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