| 产品编号 | bs-12491R |
| 英文名称 | APOA1BP/AI-BP |
| 中文名称 | 载脂蛋白A1结合蛋白抗体抗体 |
| 别 名 | AI BP; AIBP; ApoA I binding protein; Apolipoprotein A I binding protein; MGC119143; MGC119144; MGC119145; YjeF N terminal domain containing protein 1; YJEFN1; NNRE_HUMAN. |
| 研究领域 | 心血管 细胞生物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Human (predicted: Chimpanzee,Sheep,Rabbit,Cow,Pig,Dog,Mouse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APOA1BP/AI-BP: 201-288/288 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Apolipoproteins are protein components of plasma lipoproteins. Apolipoprotein A-I (ApoA1) promotes cholesterol efflux from tissues to the liver for excretion. ApoA1 is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase, which is responsible for the formation of most plasma cholesteryl esters. AI-BP (Apolipoprotein A-I-binding protein), also known as YjeF N-terminal domain-containing protein 1, is a 288 amino acid secreted protein that binds ApoA1, ApoA2 and HDL. Individuals with impaired renal function show an increased rate of AI-BP excretion, indicating that it is normally reabsorbed within the kidney tubules. AI-BP belongs to the YjeF N-terminal domain protein family, which includes proteins that are frequently involved in oogenesis and spermatogenesis. There are two isoforms of AI-BP that are produced as a result of alternative splicing events. Function: APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I. Subunit: Homodimer (By similarity). Interacts with APOA1 and APOA2. Subcellular Location: Secreted Tissue Specificity: Ubiquitously expressed, with highest levels in kidney, heart and liver. Present in cerebrospinal fluid and urine but not in serum from healthy patients. Present in serum of sepsis patients (at protein level). Post-translational modifications: Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation (By similarity). Similarity: Belongs to the nnrE/AIBP family. Contains 1 YjeF N-terminal domain. SWISS: Q8NCW5 Gene ID: 128240 Database links: Entrez Gene: 128240 Human Entrez Gene: 246703 Mouse Omim: 608862 Human SwissProt: Q8NCW5 Human SwissProt: Q8K4Z3 Mouse Unigene: 528320 Human Unigene: 205996 Mouse Unigene: 14886 Rat |
| 产品图片 |
Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Human 293T cell lysates
Lane 6: Human LO2 cell lysates
Primary: Anti-APOA1BP/AI-BP (bs-12491R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
