| 产品编号 | bs-12950R |
| 英文名称 | CRTC3 Rabbit pAb |
| 中文名称 | CREB调节转录激活因子3抗体 |
| 别 名 | CREB regulated transcription coactivator 3; CREB-regulated transcription coactivator 3; CRTC 3; CRTC3; CRTC3_HUMAN; FLJ21868; TORC 3; TORC-3; TORC3; Transducer of CREB protein 3; Transducer of regulated cAMP response element binding protein(CREB) 3; Transducer of regulated cAMP response element binding protein 3; Transducer of regulated cAMP response element-binding protein 3; Transducer of regulated CREB protein 3. |
| 研究领域 | 细胞生物 信号转导 转运蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Sheep,Cow,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 67 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRTC3/TORC3: 151-260/619 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TORC3 is a 619 amino acid protein that localizes to both the cytoplasm and the nucleus and belongs to the TORC family. Expressed in lung tissue and B and T lymphocytes, as well as in colon, brain, ovary, kidney, prostate, colon and heart, TORC3 functions as a transcriptional coactivator for CREB-1, thereby regulating the expression of CREB-activated genes, and is also thought to activate the SIK/TORC signaling pathway. TORC3 exists as multiple alternatively spliced isoforms and, in addition to its role in transcriptional activation, is thought to induce mitochondrial biogenesis, specifically in muscle cells. Function: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). Subunit: Binding, as a tetramer, through its N-terminal region,with the bZIP domain of CREB1 enhances recruitment of TAF4 to thepromoter. 'Arg-314' in the bZIP domain of CREB1 is essential forthis interaction (By similarity). Interaction with HTLV-1 TAXenhances its transcriptional activity. Interacts, via theN-terminal with the ankyrin repeats of BCL3, to form a complex withCREB1 on CRE and TxRE responsive elements and represses HTLV-1LTR-mediated transcription. Subcellular Location: Nucleus. Cytoplasm. Appears to be mainly nuclear. Tissue Specificity: Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach. Similarity: Belongs to the TORC family. SWISS: Q6UUV7 Gene ID: 64784 Database links: Entrez Gene: 64784 Human Entrez Gene: 70461 Mouse Omim: 608986 Human SwissProt: Q6UUV7 Human SwissProt: Q91X84 Mouse Unigene: 567572 Human Unigene: 392175 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
