| 产品编号 | bs-12960R |
| 英文名称 | phospho-beta Adducin (Ser713) Rabbit pAb |
| 中文名称 | 磷酸化内收蛋白抗体 |
| 别 名 | beta Adducin(phospho S713); p-beta Adducin(phospho S713); phospho-alpha Adducin(Ser726); phospho-gamma Adducin(Ser693); ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1(alpha); Adducin 1; Adducin 2(beta); Adducin 2; Adducin 3(gamma); Adducin 3; Adducin like; alpha adducin; beta adducin. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 信号转导 细胞类型标志物 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Zebrafish,Chicken) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 80 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human beta Adducin protein: TP(p-S)FL |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. Function: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit. Subunit: Heterodimer of an alpha and a beta subunit. Subcellular Location: Membrane-cytoskeleton-associated protein. Tissue Specificity: Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the aldolase class II family. Adducinbn subfamily. SWISS: P35612 Gene ID: 119 Database links: Entrez Gene: 119 Human Entrez Gene: 11519 Mouse Omim: 102681 Human SwissProt: P35612 Human SwissProt: Q9QYB8 Mouse Unigene: 188528 Human Unigene: 11353 Rat
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
