| 产品编号 | bs-13021R |
| 英文名称 | DNA polymerase mu Rabbit pAb |
| 中文名称 | DNA聚合酶μ/DNA pol μ抗体 |
| 别 名 | DNA Polymerase Mu; DNA-directed DNA polymerase mu; DNA-directed DNA/RNA polymerase mu; DPOLM_HUMAN; FLJ35482; Pol iota; Pol Mu; Polm; POLM protein; Polymerase(DNA directed) mu; Polymerase DNA directed mu; Tdt N; Tdt-N; TdtN; Terminal transferase. |
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Specific References (1) | bs-13021R has been referenced in 1 publications.
[IF=4.368] Deng et al. DNA Damage Signaling Is Required for Replication of Human Bocavirus 1 DNA in Dividing HEK293 Cells. (2017) J.Virol.Dec 16;91(1). WB&ICC ;
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| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNA polymerase mu: 261-360/494 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DNA polymerase mu shares a number of characteristics with DNA polymerase Beta as well as with terminal deoxynucleotideyltransferase. Pol mu purportedly plays a role in microhomology mediated joining and the repair of double-stranded breaks. However, unlike other DNA polymerases, which show substrate specificity for deoxynucleotides, DNA Pol mu incorporates both deoxynucleotides and ribonucleotides in a template- directed manner. This unusual capability implies a novel role for this polymerase in DNA repair. Function: Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. Subcellular Location: Nucleus. Tissue Specificity: Expressed in a number of tissues. Abundant in thymus. Similarity: Belongs to the DNA polymerase type-X family. Contains 1 BRCT domain. SWISS: Q9NP87.1 Gene ID: 27434 Database links: UniProtKB/Swiss-Prot: Q9NP87.1 |
| 产品图片 |
Sample: Raji Cell(Human)Lysate at 30 ug
Primary: Anti-DNA polymerase mu (bs-13021R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 60kD
Sample:HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-DNA polymerase mu (bs-13021R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 60kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
