| 产品编号 | bs-13118R |
| 英文名称 | EXOC2 Rabbit pAb |
| 中文名称 | 胞吐囊复合体蛋白2抗体 |
| 别 名 | exocyst complex component 2; exocyst complex component Sec5; FLJ11026; SEC5; SEC5 like 1; SEC5L1; Sec5p; EXOC2_HUMAN. |
| 研究领域 | 细胞生物 转录调节因子 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 104 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EXOC2/SEC5: 31-130/924 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The mammalian exocyst complex (sec 6/8) is a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Sec 5 is one of eight exocyst complex subunits: Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. Exocyst complex regulation depends upon Sec5 binding Ral in a GTP-dependent manner. Human Sec5 maps to chromosome 6p25.3. Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Subunit: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EXOC3L1 (By similarity). Interacts with RALA and GNEFR/DELGEF. Interaction with GNEFR occurs only in the presence of magnesium or manganese and is stimulated by dCTP or GTP. Interacts with RALB. Subcellular Location: Plasma membrane. Tissue Specificity: Widely expressed with highest levels in brain and placenta. Similarity: Belongs to the SEC5 family. Contains 1 IPT/TIG domain. SWISS: Q96KP1 Gene ID: 55770 Database links: Entrez Gene: 55770 Human Entrez Gene: 66482 Mouse SwissProt: Q96KP1 Human SwissProt: Q9D4H1 Mouse Unigene: 484412 Human Unigene: 293510 Mouse Unigene: 2869 Rat |
| 产品图片 |
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC2) Polyclonal Antibody, Unconjugated (bs-13118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
