| 产品编号 | bs-4988R |
| 英文名称 | IL18 |
| 中文名称 | 白介素18抗体 |
| 别 名 | Interleukin-18; interferon gamma-inducing factor IGIF; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL-18; IL 1g; IL1 gamma; IL18 protein; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin18; MGC12320; IL18_HUMAN. |
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Specific References (2) | bs-4988R has been referenced in 2 publications.
[IF=4.486] Linzhao Li. et al. Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity. 2021 Feb 16 WB ; Human.
[IF=2.311] Teng Ma. et al. Interleukin‑18 and ‑10 may be associated with lymph node metastasis in breast cancer. Oncol Lett. 2021 Apr;21(4):1-1 IHC ; Human.
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| 研究领域 | 肿瘤 心血管 免疫学 细胞因子 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Pig,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-18/IGIF: 101-193/193 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells.
This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions. Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Subcellular Location: Secreted. Similarity: Belongs to the IL-1 family. SWISS: Q14116 Gene ID: 3606 Database links: Entrez Gene: 3606 Human Entrez Gene: 16173 Mouse Omim: 600953 Human SwissProt: Q14116 Human SwissProt: P70380 Mouse Unigene: 83077 Human Unigene: 1410 Mouse
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| 产品图片 |
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(bs-4988R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(bs-4988R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
