| 产品编号 | bs-8997R |
| 英文名称 | Annexin VI |
| 中文名称 | 膜粘连蛋白6抗体 |
| 别 名 | Annexin VI; 67 kDa calelectrin; Annexin A6; Annexin VI p68; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; Calcium binding protein p68; Calelectrin; Calphobindin II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin20; CPB II; CPBII; Lipocortin VI; LipocortinVI; p68; P70; Protein III; ProteinIII. |
| 研究领域 | 肿瘤 细胞生物 结合蛋白 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse (predicted: Pig,Dog,Chicken,Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 67-74kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Annexin VI: 601-673/673 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype. Function: May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Tissue Specificity: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: Phosphorylated in response to growth factor stimulation. Similarity: Belongs to the annexin family. Contains 8 annexin repeats. SWISS: P08133 Gene ID: 309 Database links: Entrez Gene: 309 Human Entrez Gene: 11749 Mouse Omim: 114070 Human SwissProt: P08133 Human SwissProt: P14824 Mouse Unigene: 412117 Human Unigene: 265347 Mouse Unigene: 204053 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Liver tissue lysates Primary: Anti-Annexin VI (bs-8997R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67-74 kDa Observed band size: 75 kDa 
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Annexin VI) Polyclonal Antibody, Unconjugated (bs-8997R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
