产品编号 | bs-13215R |
英文名称 | FRG1 Rabbit pAb |
中文名称 | 面肩肱型肌营养不良症相关蛋白FRG1抗体 |
别 名 | frg1; FRG1_HUMAN; FRG1A; FSG1; FSHD region gene 1 protein; Protein FRG1. |
研究领域 | 细胞生物 信号转导 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 29 kDa |
检测分子量 | |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FRG1: 181-258/258 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq, Jul 2008]. Function: May have a role in processing of pre-rRNA or in the assembly of rRNA into ribosomal subunits. May be involved in pre-mRNA splicing. Subunit: Identified in the spliceosome C complex. Subcellular Location: Nucleus; Cajal body. Nucleus speckle. Nucleus; nucleolus. First expressed in Cajal bodies and nuclear speckles. As speckle expression diminishes, expression is seen in dense structures (dense fibrillar component and the granular component) of the nucleolus. Tissue Specificity: Expressed in adult muscle, lymphocytes, fetal brain, muscle, and placenta. Similarity: Belongs to the FRG1 family. SWISS: Q14331 Gene ID: 2483 Database links: Entrez Gene: 2483 Human Entrez Gene: 14300 Mouse Omim: 601278 Human SwissProt: Q14331 Human SwissProt: P97376 Mouse Unigene: 203772 Human Unigene: 217312 Mouse |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FRG1) Polyclonal Antibody, Unconjugated (bs-13215R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |