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Rabbit Anti-Giantin antibody (bs-13356R)
~~~促销,代码KT802~~~
~~~促销,代码KT0830~~~
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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-13356R
英文名称 Giantin
中文名称 高尔基体相关蛋白GCP372抗体
别    名 372 kDa Golgi complex associated protein; 372 kDa Golgi complex-associated protein; GCP; GCP372; Giantin; GOGB1_HUMAN; GOLGB1; Golgi autoantigen golgin subfamily a member 1; Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1; Golgi autoantigen, golgin subfamily b, macrogolgin 1; Golgi complex associated protein 372kDa; Golgi complex associated protein 372kDa; Golgi complex-associated protein 372kDa; Golgi complex-associated protein 372kDa; Golgin B1; Golgin B1, golgi integral membrane protein; Golgin subfamily B member 1; GOLIM1; Macrogolgin; Macrogolgin; MGC33154.  
研究领域 细胞生物  信号转导  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Dog, Horse, Rabbit, Hamster, Monkey, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 376kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Giantin:751-850/3259 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]

Function:
May participate in forming intercisternal cross-bridges of the Golgi complex.

Subunit:
Homodimer; disulfide-linked. Interacts with PLK3.

Subcellular Location:
Golgi apparatus membrane.

SWISS:
Q14789

Gene ID:
2804

Database links:

Entrez Gene: 2804 Human

Entrez Gene: 224139 Mouse

Entrez Gene: 192243 Rat

Omim: 602500 Human

SwissProt: Q14789 Human

Unigene: 213389 Human

Unigene: 244815 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Paraformaldehyde-fixed, paraffin embedded (human cervix cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervix cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: Lovo Cells(blue).
Primary Antibody: Rabbit Anti-Giantin/AF488 Conjugated antibody (bs-13356R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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