| 产品编号 | bs-8615R |
| 英文名称 | Ribonuclease 3/ECP |
| 中文名称 | 嗜酸性粒细胞阳离子蛋白抗体 |
| 别 名 | eosinophil cationic protein; EAR; Ear11; Ear4; EAR5; ECP; LOC192264; MGC143624; mR3; mR5; R1; R14; R15; R17; R5; R7; Ribonuclease 3; Rnase17; Rnase2; RNASE3; RNS3; ECP_HUMAN. |
 |
Specific References (4) | bs-8615R has been referenced in 4 publications.
[IF=12.05] Fujisawa, Daisuke, et al. "Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria." Journal of Allergy and Clinical Immunology (2014). IHC-F ; Human.
[IF=4.082] Yoshimasa Imoto. et al. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. Biomolecules. 2020 Nov;10(11):1568 IF ; Human.
[IF=2.36] Inada, Noriko, et al. "Histamine H 1 and H 4 receptor expression on the ocular surface of patients with chronic allergic conjunctival diseases." Allergology International (2017). IF(ICC) ; Human.
[IF=2.36] Tokunaga, Takahiro, et al. "The significant expression of TRPV3 in nasal polyps of eosinophilic chronic rhinosinusitis." Allergology International (2017). IHC-P ; Human.
|
| 研究领域 | 细胞生物 免疫学 通道蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human) |
| 产品应用 | WB=1:500-2000, ICC=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 16kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ribonuclease 3: 45-160/160 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ECP is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis, and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases Function: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity. Subcellular Location: Cytoplasmic granule. Matrix of eosinophil's large specific granule. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: P12724 Gene ID: 6037 Database links: Entrez Gene: 6037 Human Entrez Gene: 53876 Mouse Omim: 131398 Human SwissProt: P12724 Human SwissProt: O35290 Mouse Unigene: 73839 Human Unigene: 327386 Mouse Unigene: 10626 Rat |
| 产品图片 |
Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term)(Cat. #bs-8615R). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
RNASE3 Antibody (C-term) (Cat. #bs-8615R) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).
Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#bs-8615R) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
