| 产品编号 | bs-13467R |
| 英文名称 | GNG11 Rabbit pAb |
| 中文名称 | G蛋白γ11/Gγ11 抗体 |
| 别 名 | G protein gamma 11 subunit; GBG11_HUMAN; Gng11; GNGT11; Guanine nucleotide binding protein G; Guanine nucleotide binding protein gamma 11; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11; IG; Osubunit gamma 11 precursor; SG. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 G蛋白偶联受体 新陈代谢 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GNG11: 31-70/73 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Interacts with beta-1 and beta-3, but not with beta-2. Subcellular Location: Cell membrane. Tissue Specificity: Abundantly expressed in all tissues tested except for brain. Similarity: Belongs to the G protein gamma family. SWISS: P61952 Gene ID: 2791 Database links: Entrez Gene: 2791 Human Entrez Gene: 66066 Mouse Omim: 604390 Human SwissProt: P61952 Human SwissProt: P61953 Mouse Unigene: 83381 Human Unigene: 25547 Mouse Unigene: 892 Rat |
| 产品图片 |
Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GNG11 Polyclonal Antibody, Unconjugated(bs-13467R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
