| 产品编号 | bs-13515R |
| 英文名称 | GPR124 Rabbit pAb |
| 中文名称 | 肿瘤血管内皮标志物/G蛋白偶联受体124抗体 |
| 别 名 | DKFZp434C211; DKFZp434J0911; FLJ14390; G protein coupled receptor 124; G-protein coupled receptor 124; GP124_HUMAN; GPR 124; GPR124; KIAA1531; TEM 5; TEM5; Tumor endothelial marker 5; Tumor endothelial marker 5 precursor; GPCR GPR124. |
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Specific References (1) | bs-13515R has been referenced in 1 publications.
[IF=2.19] Taguchi K et al. Isolation of tumor endothelial cells from murine cancer. (2018)Journal of Immunological Methods. Nov 3. IF ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 信号转导 G蛋白偶联受体 细胞类型标志物 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 139 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 124: 351-450/1338 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tumor endothelial markers (TEMs) are abundantly expressed in the blood vessels of human solid tumors during angiogenesis and neoangiogensis. These include TEM1 (endosialin), TEM5 (G-protein coupled receptor 124) and TEM7 (plexin domain containing 1). TEMs are associated with the cell surface membrane at low levels in normal human and mouse tissues. TEM5 is a seven-pass transmembrane receptor, whereas TEM1, TEM7 and TEM8 span the membrane once. TEM5 expression is elevated during tumor angiogenesis and neoangiogenesis. TEM7 is highly expressed in tumor endothelium and neurons. Therefore, TEM5 and TEM7 may be suitable targets for the development of antiangiogenic therapies. Function: Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube. Subunit: Interacts with DLG1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 1 GPS domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q96PE1 Gene ID: 25960 Database links: Entrez Gene: 25960 Human Omim: 606823 Human SwissProt: Q96PE1 Human Unigene: 274136 Human |
| 产品图片 |
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Large intestine (Rat) Lysate at 40 ug
Lane 4: Lung (Rat) Lysate at 40 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-GPR124 (bs-13515R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 180-200 kD
Observed band size: 245 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
