| 产品编号 | bs-13538R |
| 英文名称 | GPR45 Rabbit pAb |
| 中文名称 | G蛋白偶联受体45抗体 |
| 别 名 | GPCR GPR45; G protein coupled receptor 45; High affinity lysophosphatidic acid receptor; Probable G protein coupled receptor 45; GPCR 45; PSP24 1; PSP24 alpha; PSP24; PSP24(ALPHA); PSP241; PSP24A. |
| 研究领域 | 细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR45: 251-350/372 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR45, also known as PSP24-1 or PSP24-α, is a 372 amino acid orphan receptor that is suspected to play a role in brain function. Expressed in brain, GPR45 has been detected in the basal forebrain, frontal cortex and caudate, but not in thalamus, hippocampus or putamen. Function: GPCR GPR54 is a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPR45 is an orphan receptor, which may play a role in brain function. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9Y5Y3 Gene ID: 11250 Database links: Entrez Gene: 11250 Human Omim: 604838 Human SwissProt: Q9Y5Y3 Human |
| 产品图片 |
Sample:
U251 Cell (Human) Lysate at 40 ug
U87mg Cell (human) Lysate at 40 ug
Primary: Anti- GPR45 (bs-13538R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
