| 产品编号 | bs-8877R |
| 英文名称 | Somatostatin 28 |
| 中文名称 | 生长抑素抗体 |
| 别 名 | Growth hormone release inhibiting factor; SMS_HUMAN; SMST; SST; Somatostatin 28; Somatostatin28; Somatostatin-28; Somatostatin-28; somatostatin SS28. |
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Specific References (1) | bs-8877R has been referenced in 1 publications.
[IF=6.709] Erika L Galer. et al. Cervical epidural electrical stimulation increases respiratory activity through somatostatin-expressing neurons in the dorsal cervical spine in rats. J NEUROSCI. 2022 Dec;: IF ; Rat.
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| 研究领域 | 细胞生物 发育生物学 神经生物学 干细胞 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Dog,Pig,Cow,Horse,Rabbit) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:200-800, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 3kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Somatostatin 28: 51-116/116 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Somatostatin is a regulatory hormone that is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G protein-coupled somatostatin receptors. This cyclic tetradecapeptide inhibits the secretion of many important hormones, including somatotropin (also designated growth hormone, or GH), insulin and glucagon. Somatostatin is found in both the hypothalamus and pancreas. Somatostatin is thought to be involved in the regulation of insulin synthesis. The hormone somatostatin has active 14 amino acid and 28 amino acid forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. In the cerebellum, Somatostatin-14 and Somatostatin-28 are highly expressed at birth and in the adult stage, respectively. Somatostatin affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. The gene encoding Somatostatin maps to human chromosome 3q28. Function: The cyclic tetradecapeptide somatostatin is widely distributed throughout the body and is an important regulator of endocrine and nervous system function. It exerts its biologic actions by binding to specific high affinity receptors on the cell surface. Somatostatin inhibits the release of somatotropin. Somatostatin has also been shown in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid. Somatostatin positive cells may also be present in medullary thyroid carcinomas, C cell hyperplasia, thymic and lung tumors and pulmonary small cell carcinomas. Subcellular Location: Secreted. Similarity: Belongs to the somatostatin family. SWISS: P61278 Gene ID: 6750 Database links: Entrez Gene: 396279 Chicken Entrez Gene: 6750 Human Entrez Gene: 20604 Mouse Omim: 182450 Human SwissProt: P33094 Chicken SwissProt: P61278 Human SwissProt: P60041 Mouse Unigene: 12409 Human Unigene: 34418 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Somatostatin 28) Polyclonal Antibody, Unconjugated (bs-8877R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Somatostatin 28) Polyclonal Antibody, Unconjugated (bs-8877R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Somatostatin 28) Polyclonal Antibody, Unconjugated (bs-8877R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
