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RabbitAnti-alpha smooth muscle Actin antibody (bs-10196R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
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产品编号 bs-10196R
英文名称 alpha smooth muscle Actin
中文名称 肌动蛋白α/α-SMA/α Actin抗体
别    名 alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha cardiac actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5  α-Smooth Muscle Actin; α Smooth Muscle Actin;
Specific References  (80)     |     bs-10196R has been referenced in 80 publications.
111 [IF=2.16] Xu, Liang, et al. "Total polysaccharide of Yupingfeng protects against bleomycin‐induced pulmonary fibrosis via inhibiting transforming growth factor‐β1‐mediated type I collagen abnormal deposition in rats." Journal of Pharmacy and Pharmacology (2014).  IHC-P ;  Rat.  222
111 [IF=2.88] Li, Liucheng, et al. "Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats." Phytomedicine 22.1 (2015): 111-119.  WB ;  Rat.  222
111 [IF=1.86] Qiao, Xi, et al. "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction."Nephrology (2015).  IHC-P ;  Rat.  222
111 [IF=1.72] Yan, Xuerui, et al. "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-α-Treated Rat Aortic Smooth Muscle Cells." Cardiovascular Toxicology (2015): 1-9.  Rat.  222
111 [IF=2.91] Pang, X-X., et al. "Urotensin II Induces ER Stress and EMT and Increase Extracellular Matrix Production in Renal Tubular Epithelial Cell in Early Diabetic Mice." Kidney and Blood Pressure Research 41.4 (2016): 434-449.  WB, IHC-P ;  Mouse.  222
111 [IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.  IHC-P ;  Mouse.  222
111 [IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900  IF(ICC) ;  Human.  222
111 [IF=1.953] Liu et al. Therapeutic effects of suppressors of cytokine signaling in diabetic nephropathy. (2014) J.Histochem.Cytoche. 62:119-28  WB, IHC-P ;  Mouse.  222
111 [IF=1.69] Zhou et al. Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration. (2017) Mol.Med.Re. 15:4123-4131  In Vitro ;  Human.  222
111 [IF=1.26] Kim et al. Vandetanib and ADAM inhibitors synergistically attenuate the pathological migration of EBV-infected retinal pigment epithelial cells by regulating the VEGF-mediated MAPK pathway. (2017) Exp.Ther.Me. 13:1415-1425  WB ;  Human.  222
111 [IF=1.92] Ouyang et al. Downregulation of microRNA-429 inhibits cell proliferation by targeting p27Kip1 in human prostate cancer cells. (2015) Mol.Med.Rep. 11:1435-41  WB ;  Human.  222
111 [IF=1.69] Park and Kim Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway. (2018) Mol.Med.Rep. 17:3563-3574  WB ;  Human.  222
111 [IF=5.638] Wang et al. MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2. (2017) Cell.Death.Dis. 8:e2792  WB ;  Mouse.  222
111 [IF=2.663] Zeng Y et al. Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.(2018) Lipids in Health and Disease, Nov 6;17(1):251.  IHC ;  Mouse.  222
111 [IF=0] Lu XY et al.Codonopsis tangshen Oliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with Streptozotocin. (2018)Nat Prod Bioprospect.8(6):441-451.  IHC ;  Rat.  222
111 [IF=4.427] Wu M et al. Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/transforming growth factor β type II receptor axis.(2018) Chemosphere 219  ICF ;  Human.  222
111 [IF=1.564] Liu P et al. Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats. (2019) Yonsei Med. J. 60(1)  IHC-P ;  Rat.  222
111 [IF=1.189] Cai YH et al. Riboflavin/ultraviolet A-induced collagen cross-linking in rabbit corneal scar. Int J Ophthalmol. 2019 Jan 18;12(1):46-50.  WB ;  Rabbit.  222
111 [IF=4.556] Jiang L et al. Detecting Vulnerable Atherosclerotic Plaques by 68Ga-Labeled Divalent Cystine Knot Peptide. Mol Pharm. 2019 Mar 4;16(3):1350-1357.  IF ;  Mouse.  222
111 [IF=4.302] Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2.  IHC-P&WB ;  Human&Rat.  222
111 [IF=4.302] Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.  WB ;  Human.  222
111 [IF=0] Mao Q et al. Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF‑β1/Smad/miR‑192 signaling pathway. EXPERIMENTAL AND THERAPEUTIC MEDICINE.2019 18: 3053-3061.  IHC-P ;  Rat.  222
111 [IF=3.266] Wu W et al. Yu Jin Pulvis inhibits carbon tetrachloride-induced liver fibrosis by blocking the MAPK and PI3K/Akt signaling pathways. Am J Transl Res. 2019 Sep 15;11(9):5998-6006. eCollection 2019.  WB&IHC-P ;  Mouse.  222
111 [IF=3.585] Zhao H et al. miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1. Toxicol Appl Pharmacol. 2019 Nov 14;386:114827.  WB ;  Rat.  222
111 [IF=3.414] Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.  WB ;  Human.  222
111 [IF=3.361] Zhang Y et al. Estrogen facilitates gastric cancer cell proliferation and invasion through promoting the secretion of interleukin-6 by cancer-associated fibroblasts. Int Immunopharmacol. 2019 Nov 18:105937.  WB ;  Human.  222
111 [IF=3.17] Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927.  WB ;  Mouse&Human.  222
111 [IF=5.959] Hou L et al. Nontoxic concentration of ochratoxin A decreases the dosage of cyclosporine A to induce chronic nephropathy model via autophagy mediated by toll-like receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153.  IF ;  human.  222
111 [IF=2.123] Han L et al. Interleukin-1β-Induced Senescence Promotes Osteoblastic Transition of Vascular Smooth Muscle Cells. Kidney Blood Press Res. 2020;45(2):314-330.  ICC ;  human.  222
111 [IF=2.538] Qi J et al. Isoliquiritigenin Inhibits Atherosclerosis by Blocking TRPC5 Channel Expression. Cardiovasc Ther . 2020 Apr 7;2020:1926249.  ICC ;  mouse.  222
研究领域 肿瘤  细胞生物  免疫学  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Rabbit, )
产品应用 WB=1:5000-20000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 42kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Actin alpha: 165-260/377 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha-Actin expression is limited to various types of muscle, whereas beta- and gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Subcellular Location:
Cytoplasm, cytoskeleton.

Post-translational modifications:
Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced (By similarity).

DISEASE:
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

Similarity:
Belongs to the actin family.

SWISS:
P62736

Gene ID:
59

Database links:
Entrez Gene: 101021287 Baboon

Entrez Gene: 515610 Cow

Entrez Gene: 59 Human

Entrez Gene: 11475 Mouse

Entrez Gene: 733615 Pig

Entrez Gene: 100009271 Rabbit

Entrez Gene: 81633 Rat

Omim: 102620 Human

SwissProt: P62739 Cow

SwissProt: P62736 Human

SwissProt: P62737 Mouse

SwissProt: P62740 Rabbit

SwissProt: P62738 Rat

Unigene: 500483 Human

Unigene: 213025 Mouse

Unigene: 195319 Rat

Unigene: 3114 Rat



Actin α/α-Actin 是一种具有收缩能力的微丝蛋白，a-SMA广泛分布于几乎所有的肌型细胞中。Actin-α蛋白主要用于检测骨骼肌、平滑肌、血管平滑肌、心肌和肌原性肿瘤 包括：平滑肌瘤、平滑肌肉瘤、横纹肌肉瘤以及肌上细胞和肌上皮瘤。Actin（肌动蛋白）是在所有真核细胞中都表达的高度保守的蛋白质。它们沿微管组成了细胞骨架的主要成分。肌动蛋白至少表达为6种异构形式。它在心脏、骨骼横纹肌组织和某些平滑肌组织中表达，调节其收缩功能。有报导说肌动蛋白在乳房瘤中是高度磷酸化的。肌动蛋白的功能失调也会导致某种类型的心脏病。平滑肌α肌动蛋白使人更感兴趣，因为编码它的基因是相对局限于在血管平滑肌细胞中表达的少数几个基因之一。肌动蛋白是标记平滑肌和肌上皮细胞肿瘤的有效工具。
产品图片
Sample:
Liver (Rat) Lysate at 40 ug
Urinary bladder (Rat) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
A549 (Cell) Lysate at 30 ug
Primary: Anti-alpha smooth muscle Actin (bs-10196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 46 kD
Sample:
A549(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Stomach (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Skeletal muscle(-) (Mouse) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-10196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Actin α/alpha-SMA Polyclonal Antibody, Unconjugated(bs-10196R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-alpha smooth muscle Actin antibody (bs-10196R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Blank control: NIH/3T3.
Primary Antibody (green line): Rabbit Anti-alpha smooth muscle Actin antibody (bs-10196R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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