| 产品编号 | bs-15391R |
| 英文名称 | GPR89A Rabbit pAb |
| 中文名称 | G蛋白偶联受体GPR89A蛋白抗体 |
| 别 名 | CGI-13; Golgi pH regulator A; Golgi pH regulator B; GPHR; GPHRA; GPHRB; GPR89; GPR89A; GPR89B; HSPC201; Protein GPR89A; Protein GPR89B; Putative MAPK-activating protein PM01; Putative NF-kappa-B-activating protein 90; SH120; UNQ192/PRO218; GPHRA_HUMAN. |
| 研究领域 | 细胞生物 免疫学 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR89A: 221-320/445 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GPR89A is a nearly identical copy of the GPR89B gene (MIM612806). Function: Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance. Subunit: Homotrimer. Subcellular Location: Golgi apparatus membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the Golgi pH regulator (TC 1.A.38) family. SWISS: B7ZAQ6 Gene ID: 653519 Database links: Entrez Gene: 653519 Human Entrez Gene: 67549 Mouse Omim: 612821 Human SwissProt: B7ZAQ6 Human SwissProt: Q8BS95 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-GPR89A (bs-15391R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR89A) Polyclonal Antibody, Unconjugated (bs-15391R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
