| 产品编号 | bs-15411R |
| 英文名称 | Rabbit Anti-HAPLN3 antibody |
| 中文名称 | 透明质酸及粘蛋白3抗体 |
| 别 名 | EXLD1; Extracellular link domain containing, 1; HAPLN3; HPLN3_HUMAN; HsT19883; Hyaluronan and proteoglycan link protein 3. |
| 研究领域 | 信号转导 细菌及病毒 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Horse,Chimpanzee) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HAPLN3: 51-150/360 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HAPLN3 is a 360 amino acid protein encoded by the human gene HAPLN3. HAPLN3 belongs to the HAPLN family and contains 1 Ig-like V-type (immunoglobulin-like) domain and two Link domains. HAPLN3 mediates the binding of complexes containing hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. HAPLN3 may also be involved in the formation of extracellular matrices contributing to perineuronal nets and facilitate the understanding of a functional role of these extracellular matrices. HAPLN3 is widely expressed with highest levels in spleen and placenta. Function: May function in hyaluronic acid binding. Subcellular Location: Secreted, extracellular space, extracellular matrix (By similarity). Tissue Specificity: Widely expressed with highest levels in spleen and placenta. Similarity: Belongs to the HAPLN family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains SWISS: Q96S86 Gene ID: 145864 Database links: Entrez Gene: 145864 Human SwissProt: Q96S86 Human Unigene: 447530 Human |
| 产品图片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-HAPLN3 antibody (bs-15411R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
