| 产品编号 | bs-15458R |
| 英文名称 | Hephaestin Rabbit pAb |
| 中文名称 | 亚铁氧化酶抗体 |
| 别 名 | CPL; HEPH; HEPH_HUMAN; Hephaestin. |
 |
Specific References (1) | bs-15458R has been referenced in 1 publications.
[IF=5.008] Rychtarcikova, Zuzana, et al. "Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism." Oncotarget (2016). WB ; Human.
|
| 研究领域 | 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 128 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hephaestin: 21-120/1158 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Hephaestin is a single-pass type I membrane protein that belongs to the multicopper oxidase family of proteins. Hephaestin, a copper-dependant ferroxidase protein, is crucial for iron exiting intestinal enterocytes into the circulation. It mediates the movement of iron across the basolateral membrane in conjunction with ferroportin 1. This is an important link between iron and copper metabolism in mammalian systems, as copper deficiency leads to reduced hephaestin and reduced iron absorption resulting in anemia. Hephaestin can bind six copper ions per monomer and is regulated by the homeobox transcription factor CDX2. Increased levels of iron leads to an increase in CDX2 expression and thus Hephaestin. Hephaestin is primarily detected in the intestine, but is also expressed in colon, breast, bone trabecural cells and fibroblasts. Function: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Tissue Specificity: Detected in breast, colon, bone trabecular cells and fibroblasts. Similarity: Belongs to the multicopper oxidase family. Contains 6 plastocyanin-like domains. SWISS: Q9BQS7 Gene ID: 9843 Database links: Entrez Gene: 9843 Human Omim: 300167 Human SwissProt: Q9BQS7 Human Unigene: 31720 Human Unigene: 43849 Rat
|
| 产品图片 |
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- Hephaestin (bs-15458R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 128 kD
Observed band size: 128 kD
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- Hephaestin (bs-15458R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 128 kD
Observed band size: 128 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
