| 产品编号 | bs-12592R |
| 英文名称 | GREM2 |
| 中文名称 | 骨形态形成蛋白拮抗蛋白2抗体 |
| 别 名 | BMP antagonist 2; CKTSF1B2; Cysteine knot superfamily 1; Cysteine knot superfamily 1 BMP antagonist 2; DAN domain family member 3; DAND 3; DAND3; GREM 2; Grem2; GREM2_HUMAN; Gremlin 2; Gremlin 2 cysteine knot superfamily homolog; Gremlin-2; Gremlin2; PRDC; Protein related to DAN and cerberus. |
| 研究领域 | 肿瘤 细胞生物 信号转导 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GREM2: 22-110/168 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The gremlin protein family contains antagonists of bone morphogenetic protein (BMP) signaling that are expressed in the neural crest. All family members are secreted proteins that act as BMP antagonists in embryonic explants and are expressed in the proximal airway epithelium of the lung during embryonic development. Gremlin-1 is required for early limb outgrowth and patterning in the FGF4-SHH feedback loop, while gremlin-2 binds and blocks the activity of BMP-2 and BMP-4. The dorsaling factor gremlin belongs to a novel gene family that includes the head-inducing factor Cerberus and the tumor suppressor DAN. Additionally, secreted gremlin relays the sonic hedgehog signal from the polarizing region to the apical ectodermal ridge. Function: Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner. Antagonized BMP4-induced suppression of progesterone production in granulosa cells. Subcellular Location: Secreted. Similarity: Belongs to the DAN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. SWISS: Q9H772 Gene ID: 64388 Database links: Entrez Gene: 64388 Human Entrez Gene: 23893 Mouse Omim: 608832 Human SwissProt: Q9H772 Human SwissProt: O88273 Mouse Unigene: 98206 Human Unigene: 25760 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
