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Rabbit Anti-TMEM176A  antibody (bs-13622R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-13622R
英文名称 TMEM176A
中文名称 跨膜蛋白176A抗体
别    名 GS188; HCA112; Hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188; T176A_MOUSE; Tmem176a; Transmembrane protein 176A.  
研究领域 肿瘤  细胞生物  神经生物学  干细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 26kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from MOUSE TMEM176A: 1-100/235 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 TMEM176A is a 235 amino acid multi-pass membrane protein belonging to the TMEM176 family. The gene encoding GS188 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia.

Subcellular Location:
Membrane.

Similarity:
Belongs to the TMEM176 family.

SWISS:
Q96HP8

Gene ID:
55365

Database links:

Entrez Gene: 55365 Human

Omim: 610334 Human

SwissProt: Q96HP8 Human

Unigene: 647116 Human



产品图片
Protein: HepG2(human) lysates at 30ug;
Primary: rabbit Anti-TMEM176A (bs-13622R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:26 kD
Observed band size:26 kD
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- TMEM176A (bs-13622R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 25 kD
Protein: mouse kidney lysates, 30ug;
Primary: Anti-TMEM176A (bs-13622R) at 1:300;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000;
ECL excitated the fluorescence;
Predicted band size : 24kD
Observed band size : 24kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated(bs-13622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (bs-13622R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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