| 产品编号 | bs-13660R |
| 英文名称 | SLAP2 |
| 中文名称 | SLAP2抗体 |
| 别 名 | A930009E21Rik; AI430952; C20orf156; FLJ21992; MARS; MGC49845; Modulator of antigen receptor signaling; RGD1562071; SLA 2; SLA2; SLAP 2; SLAP-2; SLAP2; SLAP2_HUMAN; Src like adapter protein 2; Src like adaptor 2; Src-like adapter protein 2; Src-like-adapter 2. |
| 研究领域 | 细胞生物 信号转导 细胞膜受体 淋巴细胞 t-淋巴细胞 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Dog,Cow,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLAP2: 101-200/261 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SLA2 is a 261 amino acid protein that exists as four alternatively spliced isoforms which localize to either the cytoplasm or to the cell membrane and contain one SH2 domain and one SH3 domain. Expressed predominately in tissues of the immune system, including thymus, spleen and lymph nodes, SLA2 functions as an adaptor protein that negatively regulates T-cell receptor (TCR) signaling and may inhibit T-cell activation. SLA2 interacts with Zap-70 and is subject to post-translational phosphorylation. The gene encoding SLA2 maps to human chromosome 20. Comprising approximately 2% of the human genome, chromosome 20 contains nearly 63 million bases that encode over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome. Function: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. Subunit: Interacts (via SH2 domain) with ZAP70 (phosphorylated) and CD3Z (phosphorylated). Interacts (via SH2 domain) with CSF1R (phosphorylated) (By similarity). Interacts (via its C-terminal domain) with CBL (phosphorylated). Subcellular Location: Cytoplasm. Tissue Specificity: Predominantly expressed in immune system, with highest levels in peripheral blood leukocytes. Expressed in spleen, thymus and lymph nodes. Expressed in T-cells as well as in monocytes, and at low level in B-cells. Also detected in placenta, prostate, skin, retina and colon. Post-translational modifications: Phosphorylated by CSF1R (By similarity). Similarity: Contains 1 SH2 domain. Contains 1 SH3 domain. SWISS: Q9H6Q3 Gene ID: 84174 Database links: Entrez Gene: 84174 Human Omim: 606577 Human SwissProt: Q9H6Q3 Human Unigene: 713578 Human |
| 产品图片 |
Sample: Epithelium (Mouse) Lysate at 40 ug
Primary: Anti- SLAP2 (bs-13660R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29kD Observed band size: 29kD 
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLAP2) Polyclonal Antibody, Unconjugated (bs-13660R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
