| 产品编号 | bs-16723R |
| 英文名称 | SH3KBP1 |
| 中文名称 | SH3结构域激酶结合蛋白1抗体 |
| 别 名 | c Cbl interacting protein; Cbl interacting protein; Cbl interacting protein of 85 kDa; Cbl-interacting protein of 85 kDa; CD2 binding protein 3; CD2-binding protein 3; CD2BP3; CIN 85; CIN85; GIG 10; GIG10; HSB 1; HSB-1; HSB1; Human Src family kinase binding protein 1; Human Src family kinase-binding protein 1; MIG 18; MIG18; Migration inducing gene 18; Migration inducing gene 18 protein; SH3 domain kinase binding protein 1; SH3 domain-containing kinase-binding protein 1; SH3BP 1; SH3K1_HUMAN; SH3KBP1; Src family kinase binding protein 1; src related kinase binding protein 1. |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 细胞膜受体 细胞粘附分子 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 73kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SH3KBP1: 201-300/665 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SHKBP1 is a 707 amino acid protein belonging to the KCTD3 family. Acting downstream of Flt-3/Flk-2, SHKBP1 interacts with CIN85. SHKBP1 contains a BTB/POZ domain, which is involved in ring canal formation and chromatin folding, and five WB repeats. SHKBP1 exists as two alternatively spliced isoforms and maps to human chromosome 19q13.2. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families and Fc receptors (FcRs). Function: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome. Cell junction > focal adhesion. Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes. Tissue Specificity: Ubiquitously expressed. Also expressed in some cancer cell lines. Post-translational modifications: Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus. Similarity: Contains 3 SH3 domains. SWISS: Q96B97 Gene ID: 30011 Database links: Entrez Gene: 30011 Human Omim: 300374 Human SwissProt: Q96B97 Human Unigene: 726365 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
