产品编号 | bs-13713R |
英文名称 | AJAP1 Rabbit pAb |
中文名称 | 粘合连接相关蛋白1抗体 |
别 名 | Adherens junction-associated protein 1; Adherens junction-associated protein 1; Ajap1; AJAP1_HUMAN; Membrane protein shrew-1; MOT8; SHREW-1; SHREW1 |
研究领域 | 肿瘤 细胞生物 信号转导 细胞粘附分子 细胞膜蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse,Rat |
产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 46 kDa |
检测分子量 | |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human AJAP1: 341-411/411 |
亚 型 | |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Plays a role in cell adhesion and cell migration. Subcellular Location: Basolateral cell membrane. Apical cell membrane. Cell junction > adherens junction. Mainly basolateral. Localization is mediated by AP1M2. Tissue Specificity: Expressed in uterus and pancreas (at protein level). Post-translational modifications: Thr-237 and Ser-239 may be phosphorylated; however as this position is probably extracellular, the in vivo relevance is not proven. SWISS: Q9UKB5 Gene ID: 55966 Database links: Entrez Gene: 55966 Human SwissProt: Q9UKB5 Human Unigene: 25924 Human |
产品图片 |
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Uterus (Mouse) Lysate at 40 ug
Lane 6: Uterus (Rat) Lysate at 40 ug
Lane 7: Pancreas (Mouse) Lysate at 40 ug
Lane 8: Pancreas (Rat) Lysate at 40 ug
Lane 9: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-AJAP1 (bs-13713R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |