产品编号 | bs-13766R |
英文名称 | NUDCD3 Rabbit pAb |
中文名称 | NUDCD3蛋白抗体 |
别 名 | KIAA1068; NudC domain containing 3; NudC domain containing protein 3; NudC like protein; NUDCD 3; NudCL; OTTHUMP00000159457; NUDC3_HUMAN; NudC domain-containing protein 3.. |
研究领域 | 细胞生物 发育生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat (predicted: Mouse,Pig) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 41 kDa |
检测分子量 | |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human NUDCD3: 161-260/361 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Nuclear migration is essential for growth, development and cellular function of multicellular organisms. NUDCD3 (NudC domain containing 3) is a 361 amino acid protein that contains one CS domain and is phosphorylated upon DNA damage by either ATR or ATM. Ubiquitously expressed, NUDCD3 has been observed to colocalize with Golgi marker proteins during interphase of mitosis in HeLa cells, and is found at low levels in brain, ovary and heart. The gene encoding NUDCD3 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. Function: NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition. Similarity: Contains 1 CS domain. SWISS: Q8IVD9 Gene ID: 23386 Database links: Entrez Gene: 23386 Human Omim: 610296 Human SwissProt: Q8IVD9 Human Unigene: 488171 Human |
产品图片 |
Sample:
Hl-60 Cell (Human) Lysate at 30 ug
Primary: Anti- NUDCD3 (bs-13766R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
Sample:
U937 Cell (Human) Lysate at 30 ug
Primary: Anti- NUDCD3 (bs-13766R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUDCD3) Polyclonal Antibody, Unconjugated (bs-13766R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |