| 产品编号 | bs-18890R |
| 英文名称 | CHD1 Rabbit pAb |
| 中文名称 | ATP依赖的解旋酶CHD1抗体 |
| 别 名 | ATP dependent helicase CHD 1; ATP dependent helicase CHD1; ATP-dependent helicase CHD1; CHD 1; CHD-1; CHD1; CHD1_HUMAN; Chromodomain helicase DNA binding protein 1; Chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; OTTHUMP00000222625. |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 197 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHD1: 151-250/1710 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Chromodomains participate in the recognition of lysine-methylated histone tails and nucleic acids. The CHD1 protein, named for its chromo-domain, ATPase helicase-like domain and DNA-binding domain, functions as an ATP-utilizing chromatin assembly factor. Unlike HP1 and Polycomb proteins that use single chromodomains to bind to their respective methylated Histone H3 tails, the two chromodomains of CHD1 cooperate to associate with one methylated H3 tail. Unique inserts within chromodomain 1 of CHD1 block the expected site of H3 tail binding seen in HP1 and Polycomb, and instead direct H3 binding to a groove at the interchromodomain junction. The human CHD1 gene maps to 5q21.1, and encodes a 1,709 amino acid deduced protein that shares 95.5% identity with the 1,711 amino acid mouse Chd1 polypeptide. Function: ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells. Subcellular Location: Nucleus. Cytoplasm. Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis. Similarity: Belongs to the SNF2/RAD54 helicase family. Contains 2 chromo domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: O14646 Gene ID: 1105 Database links: Entrez Gene: 1105 Human Omim: 602118 Human SwissProt: O14646 Human Unigene: 643465 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
