| 产品编号 | bs-14038R |
| 英文名称 | CPSF6 Rabbit pAb |
| 中文名称 | 剪切及多聚腺苷酸化特异性因子6蛋白抗体 |
| 别 名 | CFIm68; Cleavage and polyadenylation specificity factor 68 kDa subunit; Cleavage and polyadenylation specificity factor subunit 6; CPSF 68 kDa subunit; Cpsf6; CPSF6_HUMAN; HPBRII 4; HPBRII 7; Pre mRNA cleavage factor Im 68 kDa subunit; Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPBRII 4/7; Protein HPBRII-4/7. |
| 研究领域 | 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Sheep,Cow,Chicken,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 59 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CPSF6: 121-220/551 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. [provided by RefSeq, Jul 2008] Function: Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping. Subcellular Location: Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the RRM CPSF6/7 family. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q16630 Gene ID: 11052 Database links: Entrez Gene: 11052 Human Entrez Gene: 417844 Chicken Entrez Gene: 432508 Mouse Entrez Gene: 446580 Xenopus laevis Omim: 604979 Human SwissProt: Q5ZL34 Chicken SwissProt: Q16630 Human SwissProt: Q6NVF9 Mouse SwissProt: Q6DDW4 Xenopus laevis Unigene: 369606 Human Unigene: 478881 Mouse Unigene: 478884 Mouse Unigene: 479892 Mouse Unigene: 7296 Xenopus laevis |
| 产品图片 |
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- CPSF6 (bs-14038R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
