| 产品编号 | bs-14053R |
| 英文名称 | CREB5 |
| 中文名称 | 环腺苷酸应答元件结合蛋白5抗体 |
| 别 名 | cAMP response element binding protein 5; cAMP response element binding protein CRE Bpa; cAMP response element-binding protein CREBPa; cAMP responsive element binding protein 5; cAMP responsive element binding protein 5 isoform alpha; cAMP-responsive element-binding protein 5; CRE BPA; CRE-BPa; CREB 5; CREB-5; CREB5; CREB5_HUMAN; CREBPA; cyclic AMP responsive element binding protein 5; Cyclic AMP-responsive element-binding protein 5. |
| 研究领域 | 细胞生物 染色质和核信号 信号转导 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CREB5: 21-120/508 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Function: Binds to the cAMP response element and activates transcription. Subunit: Binds DNA as a homodimer or as a heterodimer with JUN or ATF2/CREBP1. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger. SWISS: Q02930 Gene ID: 9586 Database links: Entrez Gene: 9586 Human Entrez Gene: 231991 Mouse SwissProt: Q02930 Human SwissProt: Q8K1L0 Mouse Unigene: 437075 Human Unigene: 321138 Mouse Unigene: 210235 Rat |
| 产品图片 |
Sample:
Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-CREB5 (bs-14053R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 60 kDa 
Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB5 ) Polyclonal Antibody, Unconjugated (bs-14053R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (bs-14053R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
